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Berberine

Catalog No.
N1368
 
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$61.00
In stock
20mg
$55.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Extracted from Cortex Phellodendri Chinensis;Suitability:Water;Store the product in sealed,cool and dry condition

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt336.36
Cas No.2086-83-1
FormulaC20H18NO4
SynonymsBerberine Hydrochloride;Berberine Sulphate
Solubilityinsoluble in H2O; insoluble in EtOH; ≥14.95 mg/mL in DMSO
Chemical Name9,10-dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium
SDFDownload SDF
Canonical SMILES[H]C(C([H])([H])C1=C2[H])([H])[N+](C([H])=C(C(OC([H])([H])[H])=C3OC([H])([H])[H])C(C([H])=C3[H])=C4[H])=C4C1=C([H])C5=C2OC([H])([H])O5
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1]:

Cell lines

human hepatoma cell lines (HepG2 cells)

Preparation method

General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0.5, 2.5, 5, 7.5, 10, 15 μg/ml, 24 h

Applications

Berberine as a new upregulator of liver LDLR (low-density lipoprotein receptor) expression. In HepG2 cells, the effect of berberine was also dose dependent. Northern blot showed a 50% increase in LDLR mRNA in cells treated with 2.5 μg/ml of berberine and a maximal increase of four fold of control was seen with a concentration of 15 μg/ml. The effect of BBR on LDLR was further confirmed in another human hepatoma cell line, Bel-7402. BBR at 2.5 μg/ml increased the LDLR mRNA in these cells by 2.3-fold.
Animal experiment [2]:

Animal models

female golden hamsters

Dosage form

orally twice a day at 50 mg/kg/d or 100 mg/kg/d for 10 d.

Application

In hamsters, treatment of these hyperlipidemic animals with berberine by oral administration for 10 d resulted in dose-dependent decreases in both serum total cholesterol and LDL-c. After the 10-d treatment, berberine at a dose of 50 mg/kg/d reduced LDL-c by 26%, and at a dose of 100 mg/kg/d, reduced LDL-c by 42% as compared to the control animals on the same HFHC (high-fat and high-cholesterol) diet. The berberine effect was also time dependent. LDLR mRNA and protein levels were elevated in all berberine -treated hamsters in a dose-dependent manner. A 3.5-fold increase in mRNA and a 2.6-fold increase in protein in hamster livers treated with 100 mg/kg/d of berberine were detected.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Kong, W.,Wei, J.,Abidi, P., et al. Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nature Medicine 10(12), 1344-1351 (2004).

Quality Control

Quality Control & MSDS

View current batch:

Chemical structure

Berberine