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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
P. aeruginosa Strains
Preparation method
The solubility of this compound in DMSO is >6.4mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
1, 4, 11, 33 and 100 μM; 14 h
Applications
In P. aeruginosa strains, 5-Flucytosine (5-FC) inhibited pyoverdine synthesis and pvdE transcription. In a P. aeruginosa PAO1 fur mutant, 5-Flucytosine also inhibited pyoverdine production, suggesting that 5-FC could repress iron uptake genes through a Fur-independent mechanism. 5-Flucytosine down-regulated the expression of toxA and prpL genes, which was consistent with the strongly reduced ToxA and PrpL levels in culture supernatants, two major virulence factors of P. aeruginosa.
Animal models
mouse model of pulmonary infection; mice infected with an isogenic pvdS mutant
Dosage form
30 mg/kg per day; i.p.
Application
In a mouse model of pulmonary infection with P. aeruginosa, 5-FC almost completely protected mice from the P. aeruginosa lethal challenge. All mice infected with the pvdS mutant survived the challenge, suggesting the importance of PvdS as a major pathogenicity determinant in P. aeruginosa pulmonary infection. 5-FC also reduced lesions and inflammation in bronchi and pulmonary parenchyma.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1] Imperi F1, Massai F, Facchini M, et al. Repurposing the antimycotic drug flucytosine for suppression of Pseudomonas aeruginosa pathogenicity. Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7458-63.
