Description:

IC50: 0.6 and 0.7 μM for USP10 and USP13 activity, respectively

Autophagy is an important intracellular catabolic mechanism which mediates the degradation of cytoplasmic proteins and organelles. USP10 mediates the deubiquitination of p53, regulating deubiquitination the activities of USP10 and USP13 by Beclin1 provides a mechanism for Beclin1 to control the levels of p53. Spautin-1 is a novel autophagy inhibitor.

In vitro: Spautin-1 promotes the degradation of Vps34 PI3 kinase complexes via inhibiting two ubiquitinspecific peptidases, USP10 and USP13, that target the Beclin1 subunit of Vps34 complexes. Spautin-1 had no effect on the growth and survival of Bcap-37 cells under normal culture conditions but dramatically enhanced cell death in glucose-free media. Under glucose-free conditions, western blotting for LC3 further confirmed that autophagy was induced, which was inhibited by spautin-1. Similar results were obtained with MCF-7 and BT549 cells. Therefore, spautin-1 can sensitize tumor cells to apoptosis under nutritional deprived conditions [1].

In vivo: So far, there is no report to investigate the in vivo animal efficacy of spautin-1.

Clinical trial: Up to now, spautin-1 is still in the preclinical development stage.

Reference:
[1] Liu J, Xia H, Kim M, Xu L, Li Y, Zhang L, Cai Y, Norberg HV, Zhang T, Furuya T, Jin M, Zhu Z, Wang H, Yu J, Li Y, Hao Y, Choi A, Ke H, Ma D, Yuan J.? Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13. Cell. 2011 Sep 30;147(1):223-34.