天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

Home Cart 0 Sign in  

[ CAS No. 72040-63-2 ] {[proInfo.proName]}

,{[proInfo.pro_purity]}
Cat. No.: {[proInfo.prAm]}
Chemical Structure| 72040-63-2
Chemical Structure| 72040-63-2
Structure of 72040-63-2 * Storage: {[proInfo.prStorage]}

Please Login or Create an Account to: See VIP prices and availability

Cart0 Add to My Favorites Add to My Favorites Bulk Inquiry Inquiry Add To Cart

Search after Editing

* Storage: {[proInfo.prStorage]}

* Shipping: {[proInfo.prShipping]}

Quality Control of [ 72040-63-2 ]

Related Doc. of [ 72040-63-2 ]

Alternatived Products of [ 72040-63-2 ]
Product Citations

Product Details of [ 72040-63-2 ]

CAS No. :72040-63-2 MDL No. :MFCD00065502
Formula : C20H30N4O6S Boiling Point : No data available
Linear Structure Formula :- InChI Key :UVGHPGOONBRLCX-NJSLBKSFSA-N
M.W : 454.54 Pubchem ID :83874
Synonyms :
Chemical Name :2,5-Dioxopyrrolidin-1-yl 6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoate

Safety of [ 72040-63-2 ]

Signal Word:Warning Class:
Precautionary Statements:P261-P280-P305+P351+P338 UN#:
Hazard Statements:H302-H315-H319-H332-H335 Packing Group:
GHS Pictogram:

Application In Synthesis of [ 72040-63-2 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 72040-63-2 ]

[ 72040-63-2 ] Synthesis Path-Downstream   1~10

  • 1
  • N-(4-azido-2-nitrobenzoyl)-1,7-diaminoheptane [ No CAS ]
  • [ 72040-63-2 ]
  • 4-Azido-2-nitro-N-(7-{6-[5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-6-yl)-pentanoylamino]-hexanoylamino}-heptyl)-benzamide [ No CAS ]
  • 2
  • [ 72040-63-2 ]
  • 5-(3-aminopropen-1-yl)-5'-O-(β,γ-diphosphoryl-α-phosphonomethyl)-2'-deoxyuridine [ No CAS ]
  • 5-[3-(N-biotinyl-6-aminohexanoylamino)propen-1-yl]-5'-O-(β,γ-diphosphoryl-α-phosphonomethyl)-2'-deoxyuridine [ No CAS ]
  • 3
  • [ 34284-75-8 ]
  • [ 72040-63-2 ]
  • 4-(2-{6-[5-(2-oxo-hexahydro-thieno[3,4-<i>d</i>]imidazol-6-yl)-pentanoylamino]-hexanoylamino}-ethyl)-benzenesulfonyl fluoride [ No CAS ]
  • 4
  • [ 72040-63-2 ]
  • [ 9050-30-0 ]
  • biotinylated heparan sulfate [ No CAS ]
YieldReaction ConditionsOperation in experiment
With sodium hydrogencarbonate; In water; dimethyl sulfoxide; at 20.0℃; for 1h;pH 8.5; Heparan sulfate (HS) was biotinylated using biotin with extended spacer arms usingsuccinimidyl-6- (biotinamido) hexanoate(NHS-LC-Biotin) obtained from Pierce. About 0.5 ml HS solution (2 mg/ml in NaHC03, pH 8.5) was mixed with 0.05 ml of a freshly prepared solution of NHS-LC-Biotin in dimethyl sulfoxide. The mixture was incubated at room temperature for 1 hour. Unconjugated biotin was removed by centrifugation (10,000 RPM) through Microcon-3 filter (Millipore) followed by dilution with phosphate buffered saline (PBS). This procedure was repeated five times to ensure complete removal of free biotin. Unwanted aldehydes in the reaction were then quenched by incubation with one milliliter of Tris-glycine <Desc/Clms Page number 357>buffer (25mM-183 mM, pH 8. 3) at room temperature for 20 minutes. The mixture was subjected to three rounds of microfiltration as described above. Biotinylated HS (5 mg/ml in PBS) was aliquoted and storedat-20 C. To obtain maximum biotinylation, a25-fold molar excess of biotin was used. Using HABA reagent, it was determined that the ratio of HS to biotin was 1: 2. The extent of biotinylation of HS was determined using Avidin-HABA (Pierce Chemical Co). The HABA assay can be used over a wide range of pH and salt concentrations. HABA (4-hydroxyazobenzene-2'-carboxylic acid) is a dye that binds to avidin and can serve as an indicator of unoccupied binding sites. Avidin combines stoichiometrically with biotin, making it possible to use any physiochemical differences between avidin and the avidin-biotin complex as the basis of a qualitative and quantitative assay method for either component. When HABA binds to avidin, there is a large spectral change in the HABA dye. A new absorption band appears at 500 nm, which is characteristic of the quinoid form of the dye. The avidin-biotin complex does not bind HABA and because the dissociation constant of the complex is so low, the dye is stoichiometrically displaced by biotin. Consequently, the HABA assay can be the basis of both colorimetric and titrimetric assays. The amount of avidin can be calculated directly from the increased absorbance at 500 nm, or the dye may be used as an indicator in a spectrophotometric titration with biotin. The absorption band that results from the avidin-HABA complex decreases proportionately when biotin is added. Since biotin has such a high affinity for avidin, it displaces the HABA dye. The unknown amount of biotin can be determined by preparing a standard curve using known amounts of biotin to displace the HABA which bound to avidin, and plotting against the absorbance at 500 mu. HABA solution was prepared by adding 24.2 mg of HABA (Pierce) to 9.9 ml H20, and then adding0. 1 ml1 M NaOH. Avidin-HABA reagent was prepared by adding 10 mg of avidin and 600 gl of HABA solution to 19.4 ml of phosphate buffered saline. To1 ml of Avidin-HABA reagent in a cuvette,100ul of biotinylated HS was added, and the optical density was measured at 500 nm in a spectrophotometer. A standard curve was determined using known amounts of HABA. The decrease in optical density of the HABA following the addition of biotinylated HS was determined.
  • 5
  • [ 6066-82-6 ]
  • [ 72040-64-3 ]
  • [ 72040-63-2 ]
  • 6
  • [ 51857-17-1 ]
  • [ 72040-63-2 ]
  • (6-{6-[5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-4-yl)-pentanoylamino]-hexanoylamino}-hexyl)-carbamic acid tert-butyl ester [ No CAS ]
  • 7
  • [ 4443-68-9 ]
  • [ 72040-63-2 ]
  • 8
  • [ 72040-63-2 ]
  • 6-[5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-4-yl)-pentanoylamino]-hexanoic acid (6-amino-hexyl)-amide trifluoroacetate [ No CAS ]
  • 9
  • [ 72040-63-2 ]
  • [(6-{6-[5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-4-yl)-pentanoylamino]-hexanoylamino}-hexylcarbamoyl)-methyl]-carbamic acid tert-butyl ester [ No CAS ]
  • 10
  • [ 72040-63-2 ]
  • 6-[5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-4-yl)-pentanoylamino]-hexanoic acid [6-(2-amino-acetylamino)-hexyl]-amide [ No CAS ]
Recommend Products
Same Skeleton Products
Historical Records
; ;