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Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes
Wang, Jinli ; Bonacquisti, Emily E. ; Brown, Adam D. , et al. Cells,2020,9(3):660. DOI: 10.3390/cells9030660 PubMed ID: 32182815
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Abstract: A limitation of using exosomes to their fullest potential is their limited secretion from cells, a major bottleneck to efficient exosome production and application. This is especially true for mesenchymal stem cells (MSCs), which can self-renew but have a limited expansion capacity, undergoing senescence after only a few passages, with exosomes derived from senescent stem cells showing impaired regenerative capacity compared to young cells. Here, we examined the effects of small mol. modulators capable of enhancing exosome secretion from MSCs. The treatment of MSCs with a combination of N-methyldopamine and norepinephrine robustly increased exosome production by three-fold without altering the ability of the MSC exosomes to induce angiogenesis, polarizemacrophages to an anti-inflammatory phenotype, or downregulate collagen expression. These small mol. modulators provide a promising means to increase exosome production by MSCs.
Keywords: exosome secretion ; proteomics ; small molecules
Purchased from AmBeed: 59-47-2
CAS No. : | 59-47-2 | MDL No. : | MFCD00004718 |
Formula : | C10H14O3 | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | JWDYCNIAQWPBHD-UHFFFAOYSA-N |
M.W : | 182.22 | Pubchem ID : | 4059 |
Synonyms : |
Decontractyl; Cresoxydiol; Memphenesin; Mephedan
|
Chemical Name : | 3-(o-Tolyloxy)propane-1,2-diol |
Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P264-P270-P301+P312+P330-P501 | UN#: | N/A |
Hazard Statements: | H302 | Packing Group: | N/A |
GHS Pictogram: |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.