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Improved preparative enzymatic glycosylation of vancomycin aglycon and analogues
Moore, Maxwell J. ; Qin, Pengjin ; Keith, D. Jamin , et al. Tetrahedron,2023,131,133211. DOI: 10.1016/j.tet.2022.133211 PubMed ID: 36776940
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Abstract: Modifications to the enzymic glycosylation of vancomycin and its residue 4 thioamide analog are detailed that significantly reduce the enzyme loading and amount of glycosyl donor needed for each glycosylation reaction, provide a streamlined synthesis and replacement for the synthetic UDP-vancosamine glycosyl donor to improve both access and storage stability, and permit a single-pot, two-step conversion of the aglycons to the fully glycosylated synthetic glycopeptides now conducted at higher concentrations The improvements are exemplified with the two-step, one-pot glycosylation of [Ψ[C(=S)NH]Tpg4]vancomycin aglycon (92%) conducted on a 400 mg scale (2 mg-1 g scales) and vancomycin aglycon itself (5 mg scale, 84%).
Keywords: Vancomycin ; Vancomycin analogues ; Enzymatic glycosylation
Purchased from AmBeed: 27821-45-0
CAS No. : | 27821-45-0 | MDL No. : | MFCD00084679 |
Formula : | C9H12N2Na2O12P2 | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | - |
M.W : | 448.12 | Pubchem ID : | - |
Synonyms : |
UDP disodium salt
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Chemical Name : | Sodium ((2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen diphosphate |
Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P261-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H315-H319-H335 | Packing Group: | N/A |
GHS Pictogram: |