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[0232] Compound 4 was phosphorylated as schematically shown above. To a solution of compound 4 (4.62 g, 8.47 mmol) in THF (50 mL) was added di-tert-butyl N, N- diisopropylphosphoramidite (5.4 mL, 16.9 mmol) and tetrazole (1.78 g, 25.41 mmol). The reaction was stirred at room temp for 3 h then cooled to -40C and treated with a solution of 3-chloroperoxybenzoic acid (85%, 2.06 g, 10.16 mmol) in CH2C 12 (50 mL). The reaction was stirred for 15 min then partitioned between EtOAc (50 mL) and 10% aqueous Na2S203 (75 mL). The organic layer was then washed with 10% aqueous Na2S203 (50 mL), saturated NaHC03 (50 mL), and brine (75 mL). The organic layer was then dried (MgSC^), filtered, and evaporated. The crude product was purified by flash column chromatography (silica, 50% EtOAc/hexane) to yield the desired phosphate 7 (1 .27 g, 20%) as a pale-yellow oil.
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