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Postion:Product Catalog >Biochemical Engineering>Amino Acids and proteins>Tyrosinase
Tyrosinase
  • Tyrosinase

Tyrosinase NEW

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Package 5mg 50mg
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Supply Ability: 10g
Update Time: 2025-04-28

Product Details

Product Name: Tyrosinase CAS No.: 9002-10-2
Supply Ability: 10g Release date: 2025/04/28

Product Introduction

Bioactivity

NameTyrosinase
DescriptionTyrosinase (Polyphenol oxidase) is a key rate-limiting enzyme encoded by the TYR gene that controls melanin production and catalyzes tyrosine oxidation. It is mainly found in melanosomes and plays an important role in melanogenesis, plant secondary metabolism, wound healing and immune response.
In vitroTyrosinase activity assay I. Preparation of mother solution and working solution 1. Preparation of mother solution: Dissolve Tyrosinase in ddH2O and prepare it to 1 mg/mL. 2. Preparation of working solution: Dilute the mother solution to a concentration of 200-400 U/mL. Note: The mother solution can be stored in aliquots at -20℃ or -80℃, and the working solution should be prepared and used immediately. II. Experimental operation method 1. Adjust the spectrophotometer to 280 nm and 25℃ and add the sample to each colorimetric tube in the following order: 1mL 0.5 M Phosphate buffer, pH 6.5 1 mL 0.001 M L-Tyrosine 0.9 mL ddH2O 2. Fill the colorimetric tube with oxygen through the capillary for 4-5 minutes to fully oxidize the mixture in the reaction. 3. Place the colorimetric tube on the spectrophotometer and record the absorbance at 280nm for 4-5 minutes, which is recorded as the absorbance of the blank group. 4. Add 0.1 mL of appropriately diluted enzyme and record the absorbance at 280nm for 10-12 minutes. 5. Calculate 1U = the amount of enzyme that causes the absorbance at 280 nm to increase by 0.001 per minute.
StoragePowder: -20°C for 3 years | Shipping with blue ice.
Solubility InformationH2O : 40 mg/mL, Sonication is recommended.
Inhibitors RelatedAloin | 3-O-Ethyl-L-ascorbic acid | Kojic acid | Hexylresorcinol | Monobenzone | Arbutin | Oxyresveratrol | 2-Ketoglutaric acid | Glycolic acid | Methyl cinnamate | Potassium iodide | BENZYLACETONE

Company Profile Introduction

Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers. TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.

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