physalin F NEW
| Price | $45 | $89 | $129 |
| Package | 1mg | 5mg | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-15 |
Product Details
| Product Name: physalin F | CAS No.: 57423-71-9 |
| Purity: 99.61% | Supply Ability: 10g |
| Release date: 2024/11/15 |
Product Introduction
Bioactivity
| 名稱 | physalin F |
| 描述 | Physalin F is a natural blocker of CaV2.3 (R-type) and CaV2.2 (N-type) voltage-gated calcium channels.It is a secosteroid with potent anti-inflammatory and immunomodulatory activities. Physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by Human T-lymphotropic virus type 1 (HTLV-1) infection |
| 體外活性 | METHODS: Three renal cancer cell lines (A498, ACHN, and UO-31) were treated with physalin F (0, 0.3, 1, 3, and 10 μg/mL, 24 h), and cell viability was determined by MTT assay. RESULTS Physalin F inhibited cell viability in human renal cancer cell lines A498, ACHN, and UO-31 in a concentration-dependent manner with IC50 values ??of 1.40 μg/mL, 2.18 μg/mL, and 2.81 μg/mL, respectively. [3] METHODS: A498 cells were incubated in the absence or presence of physalin F (10 μg/mL, 6, 12, 18, 24 hours) for the indicated time, and the cells were harvested and prepared for detection of (A) mitochondrial membrane potential using FACScan analysis; A498 cells were incubated in the presence of physalin F (10 μg/mL, 3, 6, 8, 12, 18, 24 hours) for the indicated time, and the cells were harvested and prepared for detection of pro-caspase-8, 9, caspase-3, PARP, and p53, p21 expression using Western blotting. RESULTS The expression of Bcl-2 protein family including Bcl-2 and Bcl-xL was decreased after treatment with physalin F, and physalin F induced apoptosis of A498 cells through a mitochondria-dependent pathway; physalin F induced apoptosis by inducing p53 and p21 proteins, followed by cleavage of caspase-8/-9/-3 and PARP. [3] |
| 體內(nèi)活性 | Physalin F is a secosteroid with potent anti-inflammatory and immunomodulatory activities.?A concentration-dependent inhibition of spontaneous proliferation of PBMC from HAM/TSP subjects was observed in the presence of physalin F, as evaluated by (3)H-thymidine uptake.?The IC50 for physalin F was 0.97 0.11 μM.?Flow cytometry analysis using Cytometric Bead Array (CBA) showed that physalin F (10 μM) significantly reduced the levels of IL-2, IL-6, IL-10, TNF-α and IFN-γ, but not IL-17A, in supernatants of PBMC cultures.?Next, apoptosis induction was addressed by using flow cytometry to evaluate annexin V expression.?Treatment with physalin F (10 μM) increased the apoptotic population of PBMC in HAM/TSP subjects.?Transmission electron microscopy analysis of PBMC showed that physalin F induced ultrastructural changes, such as pyknotic nuclei, damaged mitochondria, enhanced autophagic vacuole formation, and the presence of myelin-like figures.?In conclusion, physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by HTLV-1 infection. |
| 存儲(chǔ)條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 60 mg/mL (113.95 mM) |
| 關(guān)鍵字 | cytokine | PBMC | HTLV-1 | infection | inflammatory | Inhibitor | Apoptosis | immunomodulatory | inhibit | physalin F | proliferation |
| 相關(guān)產(chǎn)品 | Stavudine | 5-Fluorouracil | Myricetin | Meclizine dihydrochloride | Dextran sulfate sodium salt (MW 4500-5500) | Sodium 4-phenylbutyrate | L-Ascorbic acid | Metronidazole | Sorafenib | Tributyrin | Salicylic acid | Oleic acid |
| 相關(guān)庫(kù) | 抗癌天然產(chǎn)物庫(kù) | 中藥單體化合物庫(kù) | 植物來(lái)源化合物庫(kù) | 天然產(chǎn)物庫(kù) | 抗病毒庫(kù) | 中藥抗炎分子庫(kù) | 高通量篩選天然產(chǎn)物庫(kù) | 抗衰老化合物庫(kù) | 抗感染天然產(chǎn)物庫(kù) | 免疫/炎癥分子化合物庫(kù) | 已知活性化合物庫(kù) | 抗病毒中藥單體化合物庫(kù) |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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