Nutlin-3a NEW
| Price | $45 | $64 | $97 |
| Package | 1mg | 2mg | 5mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-18 |
Product Details
| Product Name: Nutlin-3a | CAS No.: 675576-98-4 |
| Purity: 99.05% | Supply Ability: 10g |
| Release date: 2024/11/18 |
Product Introduction
Bioactivity
| Name | Nutlin-3a |
| Description | Nutlin-3a is the active enantiomer of Nutlin-3, an MDM2 antagonist that inhibits MDM2-p53 interaction (Ki=90 nM) and activates p53. Nutlin-3a binds preferentially to the p53-binding pocket of MDM2, leading to stabilization of p53 and activation of the p53 pathway. Nutlin-3a has antitumor activity. |
| Cell Research | SRB(Only for Reference) |
| Kinase Assay | Biacore studies: Competition assays are performed on a Biacore S51. A Series S Sensor chip CM5 is derivatized for immobilization of a PentaHis antibody for capture of the His-tagged p53. The level of capture is ~ 200 response units (1 response unit corresponds to 1 pg of protein per mm 2). The concentration of MDM2 protein is kept constant at 300 nM. Test compounds are dissolved in DMSO at 10 mM and further diluted to make a concentration series of inhibitor in each MDM2 test sample. The assays are run at 25 °C in running buffer (10 mM Hepes, 0.15 M NaCl, 2% DMSO). MDM2-p53 binding in the presence of inhibitor is calculated as a percentage of binding in the absence of inhibitor and IC50 is calculated using Microsoft Excel |
| In vitro | METHODS: N1E-115 cells were treated with Ionomycin calcium (0.2-10 μM) for 3-24 h. Cell viability was determined by trypan blue dye exclusion assay. RESULTS: Ionomycin calcium induced cell death in a concentration and time dependent manner. [1] METHODS: Fura-2-loaded mouse cerebellar astrocytes were treated with Ionomycin calcium (0.1-10 μM) for 35 min, and cell membrane Ca(2+) concentration was monitored by changes in the Fura-2 340/380 ratio. RESULTS: At concentrations ≤1 μM [Ca2+]c slowly declined after the initial peak, reaching significantly lower levels after 35 min. However, at 2 μM of ionomycin the peak level of [Ca2+]c was sustained in the plateau phase, and at concentrations >2 μM ionomycin [Ca2+]c was significantly higher after 35 min than at the initial peak. [2] |
| In vivo | METHODS: To investigate the mechanism of demyelination induced in vivo, Ionomycin calcium (2-50 μM, 0.5 μL) was dorsal column injected into SD rats. RESULTS: Rats injected with Ionomycin calcium showed varying degrees of lesions observed from days 1-21, including areas of localized edema, a small number of scattered demyelinated axons, and larger lesions containing up to 200 demyelinated or degenerated axons. [3] |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | Ethanol : 58.2 mg/mL (100 mM) DMSO : 55 mg/mL (94.58 mM) |
| Keywords | E1 activating enzyme | inhibit | E3 ligating enzyme | p53 | carcinomas | E1/E2/E3 Enzyme | ovarian | Nutlin-3 | Ubiquitin ligase | Ubiquitin conjugating enzyme | Ubiquitin activating enzyme | Nutlin 3a | Autophagy | Inhibitor | Nutlin-3a | Nutlin3a | MDM-2/p53 | Rebemadlin | E2 conjugating enzyme | Apoptosis |
| Inhibitors Related | Stavudine | 5-Fluorouracil | Sodium 4-phenylbutyrate | L-Ascorbic acid | Hydroxychloroquine | Guanidine hydrochloride | Taurine | Tributyrin | Curcumin | Paeonol | Naringin | Gefitinib |
| Related Compound Libraries | 抗肺癌化合物庫 | 細(xì)胞凋亡化合物庫 | 經(jīng)典已知活性庫 | 谷氨酰胺代謝化合物庫 | 細(xì)胞焦亡化合物庫 | 抑制劑庫 | 抗衰老化合物庫 | 已知活性化合物庫 | 抗癌化合物庫 | 抗癌活性化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $1520.00/25mg |
VIP1Y
|
TargetMol Chemicals Inc.
|
2024-10-23 | |
| $242.00/2mg |
VIP3Y
|
TargetMol Chemicals Inc.
|
2024-10-28 | |
| $25.00/1ASSAYS |
VIP6Y
|
Hebei Weibang Biotechnology Co., Ltd
|
2024-11-15 | |
| $1.00/100g |
VIP6Y
|
Career Henan Chemical Co
|
2019-12-20 |
United States- Since: 2011-01-07
- Address: 36?Washington?Street, Wellesley?Hills
INQUIRY