IWP-2 NEW
| Price | $43 | $61 | $113 |
| Package | 5mg | 10mg | 25mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: IWP-2 | CAS No.: 686770-61-6 |
| Purity: 99.76% | Supply Ability: 10g |
| Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | IWP-2 |
| Description | IWP-2 is a Wnt pathway inhibitor (IC50=27 nM) and an ATP-competitive CK1δ inhibitor (IC50=40 nM for M82FCK1δ). IWP-2 inhibits self-renewal of embryonic stem cells and has been used in stem cell and organoid research. |
| Cell Research | IWP-2 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. The human RCC cell lines 786O and Caki-2 (5×103) are seeded into 96-well plates. Cell viability is estimated by MST assay after Caki-2 acells are incubated with ncreasing concentrations of LEF together with 20 μM IWP-2 for 48 h.After treatment, 10 μL MTS is added into each well for 2 h incubation. The absorbance is measured using a model ELX800 Micro Plate Reader at 490 nm. For colony formation assay, Caki-2 cells are trypsinized to single cell suspensions and seeded into fresh 6-well plates at 1000 cells/well. Then cells are incubated with LEF at depicted concentrations for 7 days. Colonies are fixed with absolute methanol for 15 min and then stained with 0.1% crystal violet for 20 min. After washing with PBS three times, the colonies with a diameter over 2 mm are visualized by a digital camera[2]. |
| In vitro | METHODS: Human gastric cancer cells MKN28 were treated with IWP-2 (5-50 μM) for 1-5 days, and cell proliferation was detected by CellTiter Assay. RESULTS: After four days of treatment in MKN28 cell line, 10-50 μM IWP-2 significantly inhibited the proliferation of MKN28 cells. [1] METHODS: L-Wnt3A cells were treated with IWP-2 (5 μM) for 24 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: IWP-2 blocked all detected Wnt-dependent biochemical changes, phosphorylation of Lrp6 receptor and Dvl2, and accumulation of β-catenin. [2] |
| In vivo | METHODS: To study the effects on hair loss, C57/BL6 mice were treated with AA antioxidant (100 mg/mL, topically applied to the skin), PBMT, and IWP-2 (20 mg/kg, i.v., every two days). RESULTS: PBMT promoted hair regrowth after resting shaving stimulation. Importantly, the use of AA and IWP-2 eliminated the differences in HF growth rates induced by PBMT in vivo. [3] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : < 1 mg/ml |
| Keywords | Casein Kinase | Wnt | Inhibitor | IWP-2 | IWP 2 | Porcupine | inhibit |
| Inhibitors Related | Urea | TBCA | Wnt pathway activator 1 | Ellagic acid | CHIR-99021 | Emodin | XAV-939 | LY-364947 | Nefopam hydrochloride | KY-05009 | Bisdemethoxycurcumin | LY294002 |
| Related Compound Libraries | Bioactive Compound Library | Cancer Cell Differentiation Compound Library | Kinase Inhibitor Library | Anti-Obesity Compound Library | Anti-Ovarian Cancer Compound Library | Neuroprotective Compound Library | Inhibitor Library | Metabolism Compound Library | Bioactive Compounds Library Max | Anti-Cancer Compound Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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