H2DCFDA NEW
| Price | $37 | $50 |
| Package | 50mg | 100mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-18 |
Product Details
| Product Name: H2DCFDA | CAS No.: 4091-99-0 |
| Purity: 99.47% | Supply Ability: 10g |
| Release date: 2024/11/18 |
Product Introduction
Bioactivity
| 名稱 | H2DCFDA |
| 描述 | H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) with cell membrane permeability. |
| 體外活性 | METHODS: Flow cytometry was used to detect ROS levels: 1, H2DCFDA was dissolved into 10 mM DMSO stock solution and further diluted with PBS before use. 2. Adherent cells are incubated with 5 μM H2DCFDA solution for 30 min at 37°C, protected from light, then harvested with 0.05% trypsin-EDTA solution, suspended in fresh medium and immediately analyzed by flow cytometry (488 nm). [1] METHODS: Confocal microscopy was performed to detect ROS levels: 1. H2DCFDA was dissolved into 10 mM DMSO stock solution and further diluted with PBS before use. 2. Coverslips containing cells were placed in 5 μM H2DCFDA staining solution and incubated for 60 min at 37°C, protected from light, then washed and imaged with a confocal laser scanning microscope Leica TCS SL equipped with an argon laser. [1] |
| 體內(nèi)活性 | METHODS: Fluorescence microscopy was used to analyze the oxidative activity of LPS induced peritonitis in mice: 1, H2DCFDA was dissolved in 100 μL ethanol and further diluted with PBS before use. 2. C57BL/6J mice were injected intraperitoneally with LPS (0.1-1 mg/mL) to induce peritonitis. 3.5 h later, H2DCFDA (0.1-0.8 mg/ml) was injected intraperitoneally. 3. 30 min after H2DCFDA injection, the animals were killed by cervical dislocation, and the peritoneal cells were recovered by rinsing with 5 mL of ice-cold HBSS solution at pH 7.4. 4. Peritoneal cells were washed and resuspended in PBS. Macrophages were removed by adhesion method after incubation in a polystyrene dish at 37℃ for 30 min. The supernatant was recovered and approximately 20,000-25,000 leukocytes were added to microscope slides using a cytocentrifuge. The slides were then analyzed using fluorescence microscopy. [2] |
| 存儲條件 | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | Ethanol : 14.29 mg/mL (29.33 mM), Sonication is recommended. DMSO : 50 mg/mL (102.61 mM) |
| 關鍵字 | Reactive Oxygen Species | inhibit | 2',7'-Dichlorodihydrofluorescein Diacetate | Inhibitor | H-2DCFDA | H2DCFDA | 2',7'-Dichlorodihydrofluorescein |
| 相關產(chǎn)品 | Imeglimin hydrochloride | Acetylcysteine | L-Ascorbic acid |
| 相關庫 | 銅死亡化合物庫 | 經(jīng)典已知活性庫 | 抗卵巢癌化合物庫 | 抗乳腺癌化合物庫 | 抗前列腺癌化合物庫 | 抗衰老化合物庫 | 已知活性化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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