GANT 61 NEW
| Price | $33 | $52 | $81 |
| Package | 2mg | 5mg | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: GANT 61 | CAS No.: 500579-04-4 |
| Purity: ≥95% | Supply Ability: 10g |
| Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | GANT 61 |
| Description | GANT 61 (GANT61) is an inhibitor for Gli1 and Gli2. |
| Cell Research | BrdU Incorporation Assay. Subconfluent cells are grown in reduced FBS (2.5%) for 48 h in the presence of 5 μM test compound (or DMSO) on white 96-well plates with clear bottom. Subsequently, cells are labeled for 2 h with BrdU, fixed, and analyzed.(Only for Reference) |
| Kinase Assay | Dual Luciferase Assay: HEK293 cells are transfected with GLI1 expression plasmid, together with the reporter plasmids 12×GliBSLuc and R-Luc on 10-cm plates (day 0). Twenty-four hours later, cells are seeded in white 96-well plates with clear bottom at a density of 15,000 cells per well. Cells are allowed to attach, and compounds are added at a final concentration of 10 μM in DMSO (0.5% final DMSO concentration) (day 1.5). Cells are grown for another 24 h, subsequently lysed, and then analyzed by using the Dual Luciferase kit. |
| In vitro | Nude mice bearing SK-N-AS neuroblastoma xenografts administered orally with 50 mg/kg GANT61 exhibited significant tumor growth inhibition on day 12 of the experiment, achieving a 63% reduction in tumor volume compared to the control group. Additionally, in nude mice injected with GLI1-positive 22Rv1 prostate cancer cells, GANT61 induced tumor regression to the point where no discernible tumors were observed. |
| In vivo | GANT61 effectively inhibits tumor cell proliferation in vitro through a GLI-dependent mechanism. At a concentration of 30μM, GANT61 halts growth and induces apoptosis in acute myeloid leukemia cells. It exhibits potent cytotoxicity towards human colon cancer cell lines, eradicating colony formation. GANT61 specifically targets chronic lymphocytic leukemia cells over normal B lymphocytes, triggering apoptosis. In the early S phase of human colon cancer cell lines, GANT61 induces DNA replication inhibition leading to DNA damage signaling through the ATM-Chk2 axis and consequent cell death. Furthermore, GANT61 inhibits GLI1's DNA binding capability and suppresses the hedgehog signaling pathway with an IC50 of 5μM, displaying higher selectivity over other pathways (such as TNF signaling/NFκB activation, glucocorticoid receptor gene transcription, and the Ras-Raf-Mek-Mapk cascade). |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 4.3 mg/mL (10 mM), Sonication is recommended. Ethanol : 43 mg/mL (100 mM) |
| Keywords | Autophagy | Inhibitor | Gli | GANT 61 | GANT-61 | NSC-136476 | NSC136476 | inhibit |
| Inhibitors Related | Stavudine | Xylitol | Myricetin | Sodium 4-phenylbutyrate | Hydroxychloroquine | Guanidine hydrochloride | Taurine | Curcumin | Oxyresveratrol | Paeonol | Naringin | Gefitinib |
| Related Compound Libraries | Reprogramming Compound Library | Bioactive Compound Library | Membrane Protein-targeted Compound Library | Autophagy Compound Library | Anti-Obesity Compound Library | Inhibitor Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | GPCR Compound Library | Anti-Cancer Compound Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36?Washington?Street, Wellesley?Hills
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