FITC-Dextran (MW 10000) NEW
| Price | $32 | $53 | $83 |
| Package | 5mg | 10mg | 25mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: FITC-Dextran (MW 10000) | CAS No.: 60842-46-8 |
| Supply Ability: 10g | Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | FITC-Dextran (MW 10000) |
| Description | FITC-Dextran (MW 10000) is a marker (10 kDa) formed by coupling FITC to dextran. FITC-Dextran is a labeled polysaccharide composed of branched glucose molecules of various lengths, enabling the determination of solute, ionic, and protein permeability of the blood-brain barrier depending on the size of the dextran used. |
| In vitro | METHODS: FITC-dextran assay for RBL-2H3 mast cell line: 1. Mix 1 mg of FITC-dextran powder per 1 mL of medium. Use a cellulose acetate syringe filter with a pore size of 0.22 μm to filter the dissolved FITC-dextran. 2. Add 10 μL of cell suspension to a chamber coverslip pre-filled with fresh FITC-dextran supplemented medium. 3. Incubate RBL cells overnight at 37°C in a humidified environment with 5% CO2. Cells should be maintained at a subfusion level to ensure cell separation and that each cell can be easily identified individually under a microscope. 4. Wash the chamber coverslips three times by aspirating the medium from the chamber and refilling with 300 μL of Tyrode Buffer preheated to 37°C. The coverslips should be cleaned with the Tyrode Buffer. Finally, replenish the chamber with 300 μL of Tyrode Buffer preheated to 37°C. 5. Select the region of interest to be tracked by turning on the fluorescent light source and selecting an appropriate fluorescent filter, choose a filter with green fluorophores to view FITC-dextran. if using a laser-based microscope, turn on the 488 nm laser, the emission should be centered around 500-550 nm. [1] |
| In vivo | METHODS: Intestinal permeability was determined by FITC-dextran: 1. FITC-dextran was dissolved in sterilized PBS at a concentration of 80 mg/mL. 2. Mice were fasted for 4 h prior to FITC-dextran injection, followed by oral administration of 100 μL of FITC-dextran. 3. After 4 h, mouse blood is collected and centrifuged at 4000 rpm for 15 min. Plasma samples are collected in clear EP tubes and placed at 4°C, protected from light. 4. Standard FITC-dextran samples were diluted to 0-100 μg/mL with PBS and transferred to a black 96-well plate. 5. Measure the fluorescence at 485 nm excitation wavelength and 528 nm emission wavelength using a microplate reader. [2] |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | H2O : 50 mg/mL, Sonication is recommended. |
| Keywords | FITC Dextran (MW 10000) | FITCDextran (MW 10000) |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
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