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Postion:Product Catalog >Biochemical Engineering>Biochemical Reagents>Biological Dyes>Ethidium bromide
Ethidium bromide
  • Ethidium bromide
  • Ethidium bromide

Ethidium bromide NEW

Price $8 $5 $1
Package 1kg 25kg 100kg
Min. Order: 1kg
Supply Ability: g-kg-tons, free sample is available
Update Time: 2024-04-05

Product Details

Product Name: Ethidium bromide CAS No.: 1239-45-8
Min. Order: 1kg Purity: 99%
Supply Ability: g-kg-tons, free sample is available Release date: 2024/04/05
Lead time: In stock Packaging: bottle/drum/bucket/IBC, as request.
Delivery: By sea, by air, by express Origin: Manufacturer
NAME: Sun

1. Product information

Names

Nameethidium bromide
SynonymMore Synonyms

 Ethidium bromide Biological Activity

DescriptionEthidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Related Catalog
Research Areas >> Others
Signaling Pathways >> Others >> Others
In VitroGuidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The preparation of agarose gel: 1． Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated. 2． Mix the agarose powder with 1X TAE/TBE. 3． Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil). 4． Make the solution cool down before solidification. 5． Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 μL stock/100 mL gel). 6． Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light. CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
References

[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84.

[2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.

 Chemical & Physical Properties

Melting Point260-262 °C (dec.)(lit.)
Molecular FormulaC21H20BrN3
Molecular Weight394.308
Flash Point>100°C
Exact Mass393.084045
PSA55.92000
LogP2.29820
Index of Refraction1.4575 (20oC)
Storage condition2-8°C
StabilityStable. Incompatible with strong oxidizing agents.
Water Solubility40 g/L (25 oC)

 MSDS

Ethidium bromide MSDS(Chinese)

 Toxicological Information

CHEMICAL IDENTIFICATION

  • RTECS NUMBER :

  • SF7950000

  • CHEMICAL NAME :

  • Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide

  • CAS REGISTRY NUMBER :

  • 1239-45-8

  • LAST UPDATED :

  • 199712

  • DATA ITEMS CITED :

  • 79

  • MOLECULAR FORMULA :

  • C21-H20-N3.Br

  • MOLECULAR WEIGHT :

  • 394.35

  • WISWESSER LINE NOTATION :

  • T B666 HKJ EJ H2 IR& LZ &E &9/26

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

  • TYPE OF TEST :

  • LDLo - Lowest published lethal dose

  • ROUTE OF EXPOSURE :

  • Intraperitoneal

  • SPECIES OBSERVED :

  • Rodent - mouse

  • DOSE/DURATION :

  • 20 mg/kg

  • TOXIC EFFECTS :

  • Tumorigenic - active as anti-cancer agent

  • TYPE OF TEST :

  • LD50 - Lethal dose, 50 percent kill

  • ROUTE OF EXPOSURE :

  • Subcutaneous

  • SPECIES OBSERVED :

  • Rodent - mouse

  • DOSE/DURATION :

  • 110 mg/kg

  • TOXIC EFFECTS :

  • Details of toxic effects not reported other than lethal dose value

  • TYPE OF TEST :

  • Sex chromosome loss and nondisjunction

  • TYPE OF TEST :

  • Specific locus test

  • TYPE OF TEST :

  • Dominant lethal test

  • TYPE OF TEST :

  • Micronucleus test

  • TYPE OF TEST :

  • DNA inhibition

  • TYPE OF TEST :

  • Sister chromatid exchange

MUTATION DATA

  • TEST SYSTEM :

  • Bird - chicken

  • DOSE/DURATION :

  • 847 ug/kg

  • REFERENCE :

  • 47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - A1168 No. of Facilities: 17 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 34 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - A1168 No. of Facilities: 7 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 21 (estimated)

 Safety Information

SymbolArticle illustration Article illustration
GHS06, GHS08
Signal WordDanger
Hazard StatementsH302-H330-H341
Precautionary StatementsP260-P281-P284-P310
Personal Protective EquipmentEyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges
Hazard CodesT+:Verytoxic;
Risk PhrasesR21/22;R26;R36/37/38;R68
Safety PhrasesS36/37-S45-S36/37/39-S28A-S26-S22
RIDADRUN 2811 6.1/PG 1
WGK Germany3
RTECSSF7950000
Packaging GroupI
Hazard Class6.1
HS Code29339990

 Synthetic Route

Previous 1/6 Next
Article illustration

5-ethyl-3,8-din...

93840-65-4

~58%

Article illustration

Ethidium bromid...

1239-45-8

Literature: Osadchii; Shubin; Kozlova; Varlamenko; Filipenko; Boyarskikh Russian Journal of Applied Chemistry, 2011 , vol. 84, # 9 p. 1541 - 1548
Article illustration

3,8-bis((ethoxy...

52671-22-4

~59%

Article illustration

Ethidium bromid...

1239-45-8

Literature: Lion, C.; Boukou-Poba, J. P.; Charvy, C. Bulletin des Societes Chimiques Belges, 1990 , vol. 99, # 3 p. 171 - 181
Article illustration

4-(4-aminopheny...

2243-78-9

~%

Article illustration

Ethidium bromid...

1239-45-8

Literature: Bulletin des Societes Chimiques Belges, , vol. 99, # 3 p. 171 - 181

 Precursor & DownStream

Precursor  9

Previous 1/3 Next

  • Article illustration CAS#:93840-65-4
    5-ethyl-3,8-din...

  • Article illustration CAS#:52671-22-4
    3,8-bis((ethoxy...

  • Article illustration CAS#:2243-78-9
    4-(4-aminopheny...

  • Article illustration CAS#:71857-96-0
    amino-2 bis(eth...

DownStream  1


  • Article illustration CAS#:104821-25-2
    Dihydroethidium

 Customs

HS Code2933990090
Summary2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

2. Packaging

For powders: normal is 25kgs/Drum or bag, or larger/smaller package as request.

For liquids: normal 25kgs/drum, 180-300kgs/bucket, or IBC, determined by the nature of the product. 

                     Or smaller package 1kg/bottle, 10kgs/bottle as request. 


Article illustrationArticle illustration


3. Shipping

Article illustration


4. Contact information

For more details, pls contact us freely.

Email address : sun@fdachem.com

Mob: 86 13526505137

WhatsApp/Skype/Wechat/LINE: 86 13526505137





Company Profile Introduction

Henan Fengda Chemical Co., Ltd. is located in the High-tech Development Zone of Henan Province. Specializing in the production and sales of various fine chemical products required for industrial production, including chemical raw materials, organic raw materials, petrochemicals, chemical reagents, solvents, catalysts, and additives, etc.

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