天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

Welcome to chemicalbook!
+1 (818) 612-2111
Inquriy
Try our best to find the right business for you.
Do not miss inquiry messages Please log in to view all inquiry messages.

Welcome back!

RFQ
skype
MY Account
Top
Postion:Product Catalog >API>Antineoplastic agents>Tinib Antineoplastic drugs>Dasatinib monohydrate
Dasatinib monohydrate
  • Dasatinib monohydrate

Dasatinib monohydrate NEW

Price $33 $33 $50 $50 $66 $66
Package 50mg 50mg 100mg 100mg 200mg 200mg
Min. Order:
Supply Ability: 10g
Update Time: 2024-11-19

Product Details

Product Name: Dasatinib monohydrate CAS No.: 863127-77-9
Purity: 99.89% Supply Ability: 10g
Release date: 2024/11/19

Product Introduction

Bioactivity

NameDasatinib monohydrate
DescriptionDasatinib monohydrate (BMS-354825 Monohydrate) is an orally bioavailable synthetic small molecule-inhibitor of SRC-family protein-tyrosine kinases. Dasatinib monohydrate binds to and inhibits the growth-promoting activities of these kinases. Apparently, because of its less stringent binding affinity for the BCR-ABL kinase, Dasatinib monohydrate has been shown to overcome the resistance to imatinib of chronic myeloid leukemia (CML) cells harboring BCR-ABL kinase domain point mutations.
Cell ResearchBa/F3 cell lines are seeded in triplicate and incubated with escalating concentrations of Dasatinib for 72 hours. Proliferation is measured using a methanethiosulfonate-based viability assay. IC50 and IC90 values are reported as the mean of three independent experiments done in quadruplicate. The inhibitor concentration ranges are 0 nM to 32 nM (Dasatinib). The Dasatinib concentration range is extended to 200 nM for mutant T315I.(Only for Reference)
Kinase AssayKinase autophosphorylation assays: Kinase assays using wild-type and mutant glutathione S-transferase (GST)-Abl fusion proteins (c-Abl amino acids 220-498) are done. GST-Abl fusion proteins are released from glutathione-Sepharose beads before use; the concentration of ATP is 5 μM. Immediately before use in kinase autophosphorylation and in vitro peptide substrate phosphorylation assays, GST-Abl kinase domain fusion proteins are treated with LAR tyrosine phosphatase. After 1-hour incubation at 30 °C, LAR phosphatase is inactivated by addition of sodium vanadate (1 mM). Immunoblot analysis comparing untreated GST-Abl kinase to dephosphorylated GST-Abl kinase is routinely done using phosphotyrosine-specific antibody 4 g10 to confirm complete (>95%) dephosphorylation of tyrosine residues and c-Abl antibody CST 2862 to confirm equal loading of GST-Abl kinase. The Dasatinib concentration range is extended to 1,000 nM for mutant T315I. These same inhibitor concentrations are used for the in vitro peptide substrate phosphorylation assays. The three inhibitors are tested over these same concentration ranges against GST-Src kinase and GST-Lyn kinase.
In vitroIn TgE mice, Dasatinib specifically inhibits the formation of bone marrow B-cell colonies expressing LMP2A and reduces spleen size. Compared to the control Tg6/λ-MYC mice, the Dasatinib-treated group exhibited significantly smaller spleen masses. Dasatinib also suppresses lymph node enlargement in MYC/LMP2A double transgenic mice and reverses splenomegaly. When tumor cells from LMP2A/MYC double transgenic mice are transplanted into Rag1KO mice, Dasatinib can reverse the splenomegaly. Additionally, Dasatinib inhibits Lyn phosphorylation in B lymphoma cells expressing LMP2A.
In vivoDasatinib effectively inhibits wild-type Abl kinase and all its mutants (except T315I) by directly targeting all mutant Abl kinase domains and inhibiting substrate and autophosphorylation in a concentration-dependent manner. Compared to Imatinib, Dasatinib demonstrates enhanced inhibitory efficacy on the proliferation of Ba/F3 cells expressing Bcr-Abl (wild-type and all mutants except T315I), exhibiting 325 times the potency against cells expressing wild-type Bcr-Abl. After treatment with Dasatinib, the percentage of the TgE bone marrow cell colonies was reduced from 100% to 4.12%. There's a statistically significant difference in the colony formation percentage of WT and TgE bone marrow cells under Dasatinib treatment. It also inhibits the survival and proliferation of B lymphocytes induced by LMP2A expression. Additionally, Dasatinib suppresses Src signaling in certain thyroid cancer cells, inhibiting cell growth and inducing apoptosis and cell cycle arrest. A 3-day treatment with Dasatinib (0.019 μM~1.25 μM) inhibits approximately 50% growth in BCPAP, C643, SW1736, and TPC1 cell lines, with higher concentrations required to inhibit K1 cell line growth. In BCPAP, SW1736, and K1 cells, Dasatinib (at 10 nM or 50 nM) increases G1 phase cells by 9-22% while correspondingly decreasing S phase cells by 7-18%.
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility InformationEthanol : < 1 mg/mL (insoluble or slightly soluble)
H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 55 mg/mL (108.69 mM)
Keywordsorally | Bcr-Abl | Dasatinib | Apoptosis | Dasatinib monohydrate | prostate | inhibit | Autophagy | CML | BMS 354825 Monohydrate | K562 | Inhibitor | BMS-354825 | colon | antiproliferative | PC3 | WiDr | BMS354825 Monohydrate | Src | breast | Dasatinib Monohydrate | BMS 354825 | active | antitumor | BMS354825
Inhibitors RelatedStavudine | Sodium 4-phenylbutyrate | L-Ascorbic acid | Hydroxychloroquine | Guanidine hydrochloride | Tributyrin | Paeonol | Naringin
Related Compound LibrariesTyrosine Kinase Inhibitor Library | Kinase Inhibitor Library | EMA Approved Drug Library | Anti-Cancer Clinical Compound Library | Drug Repurposing Compound Library | FDA-Approved Drug Library | Anti-Cancer Approved Drug Library | Bioactive Compounds Library Max | Anti-Cancer Drug Library | Anti-Cancer Active Compound Library

Company Profile Introduction

Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers. TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.

You may like

Recommended supplier

Product name Price   Suppliers Update time
$33.00/50mg
VIP5Y
TargetMol Chemicals Inc.
2024-11-19
$5.00/1KG
VIP4Y
Hebei Chuanghai Biotechnology Co,.LTD
2024-08-21
$0.00/1g
VIP1Y
BEIJING SJAR TECHNOLOGY DEVELOPMENT CO., LTD.
2024-08-02
$1.00/1g
VIP1Y
Apeloa production Co.,Limited
2024-06-19
$120.00/1kg
VIP1Y
Hebei Zhuanglai Chemical Trading Co.,Ltd
2024-05-16
$32.00/1KG
VIP2Y
Henan Fengda Chemical Co., Ltd
2024-04-15
$0.00/1kg
VIP1Y
Shaanxi TNJONE Pharmaceutical Co., Ltd
2024-04-12
$10.00/1kg
VIP1Y
Wuhan Xinhao Biotechnology Co., Ltd
2024-03-11
$0.00/1kg
Nanjing Fred Technology Co., Ltd
2023-12-20
$0.00/1kg
VIP2Y
SHANDONG BOYUAN PHARMACEUTICAL CO., LTD.
2023-09-07
  • Since: 2011-01-07
  • Address: 36?Washington?Street, Wellesley?Hills
INQUIRY