Cytochalasin B NEW
| Price | $228 |
| Package | 1mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-11 |
Product Details
| Product Name: Cytochalasin B | CAS No.: 14930-96-2 |
| Purity: 98.79% | Supply Ability: 10g |
| Release date: 2024/11/11 |
Product Introduction
Bioactivity
| 名稱 | Cytochalasin B |
| 描述 | Cytochalasin B is a mycotoxin binding to the barbed end of actin filaments. It can disrupt the formation of actin polymers (Kd: 1.4-2.2 nM for F-actin). |
| 細胞實驗 | The attached cell lines M109c, B16BL6, and B16F10 are seeded at 1 to 4?×?10^4 cells/mL in 2 mL volumes in 24-well culture plates 1 day prior to treatment with Cytochalasin B. The suspension culture of P388/ADR cells is seeded at 5?×?10^4 cells/mL and allowed to grow overnight before Cytochalasin B treatment. Cells are treated with Cytochalasin B for 3 h, as well as 2, 3, or 4 days. In the case of continuous exposure for 2, 3, or 4 days, attached cells are trypsinized and counted with a hemacytometer. Leukemia cell suspensions are counted with a Coulter Counter. In the case of short-term exposure, cells are washed twice with fresh medium, then trypsinized (except for P388/ADR cells), reseeded, and allowed to regrow for 3 days, at which time they are counted. Growth results are calculated as the number of cells generated above the seeding density compared to the untreated control cells and graphically presented as a percent of control increase [3]. |
| 動物實驗 | For chemotherapy testing, Balb/c mice under isoflurane anesthesia are challenged with 2?×?10^5 trypan blue negative P388/S or P388/ADR cells subcutaneously (s.c.) in a volume of 200 μL. Untreated mice are kept in order to determine the lethality of the challenge without chemotherapeutic intervention. Long-term survival is defined as challenged mice that survive the duration of the observation period. Cytochalasins B and D are prepared in suspension form in 2 % carboxymethyl cellulose 1 % tween 20 (CMC/Tw) for intraperitoneal (i.p.) administration. The congeners or the vehicle are administered to leukemia-challenged mice on Days 1-8 following the initial challenge [2]. |
| 體外活性 | Cytochalasin B is a cell-permeable mycotoxin that binds to the barbed end of actin filaments, inhibiting their elongation and shortening, with Kds of 2.2 nM and 1.4 nM for F-actin in the presence of MgCl2 (2 mM) or MgCl2 (2 mM) plus KCl, respectively [1]. At 6 μM, it increases the myofibrillar fragmentation index by intensively breaking myofibrillar proteins into short segments and accelerates actin filament disruption and F-actin to G-actin transformation, thereby reducing F-actin content and significantly increasing G-actin bands during postmortem conditioning [2]. Cytochalasin B exhibits inhibitory effects on multiple murine cancer cell lines, with IC50s of 2.56 μM (M109c), 10.46 μM (B16BL6), 105.5 μM (P388/ADR), 51.9 μM (P388/S) and IC80s of 12.23 μM (M109c), 44.86 μM (B16BL6), 188.4 μM (P388/ADR), 84.1 μM (P388/S) after 3-hour treatment, and IC50s of 0.25 μM (M109c), 0.37 μM (B16F10), 0.87 μM (B16BL6), and IC80s of 0.75 μM (M109c), 1.21 μM (B16F10) after a 4-day treatment [3]. |
| 體內活性 | Cytochalasin B, administered intraperitoneally at doses of 10, 25, and 50 mg/kg, enhances the lifespan of Balb/c mice afflicted with P388/ADR leukemias in a dose-dependent manner. Specifically, a 50 mg/kg dose of Cytochalasin B results in a 10% increase in long-term survival rates for mice with multidrug-resistant P388/ADR leukemia, and a 40% increase in long-term survival for those with the drug-sensitive P388/S variant [3]. |
| 存儲條件 | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | Ethanol : 20 mg/mL (41.7 mM) DMSO : 20 mg/mL (41.7 mM) |
| 關鍵字 | Cytochalasin B |
| 相關庫 | 稀有天然產物庫 | 高通量篩選天然產物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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