Product Details
| Product Name:
C12FDG |
CAS No.:
138777-25-0 |
| Purity:
99.84% |
Supply Ability:
10g |
| Release date:
2025/06/17 |
Product Introduction
Bioactivity
| Name | C12FDG |
| Description | C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry. |
| Cell Research | Fluorescence aging-related β-galactosidase (SA-β-Gal) experiment
I. Solution preparation:
1. Mother solution preparation: Prepare a certain concentration of C12FDG mother solution, store it at -20℃ or -80℃ in the dark after aliquoting.
2. Working solution preparation: Select the appropriate working solution concentration according to the experimental requirements, and try to prepare it before use.
II. Operation steps:
1. Culture cells with a density of 105 cells/mL
2. Wash the cells once with 200 μL PBS, and then fix them with 100 μL fixative (2% formaldehyde/0.2% glutaraldehyde distilled water) at room temperature for 5 minutes.
3. Wash the cells twice with 200 μL PBS, stain with 100 μL 33 μM C12FDG (PBS, pH = 6.0) for 10 min, and then stain with 200 μL Hoechst solution (1 μg/mL Hoechst 33342) in PBS (pH 6.0) for 10 min.
d. Image the cells using a 20x objective and excitation filters of 360 nm (Hoechst 33342) and 480 nm (C12FDG), and monitor the cells using emission filters of 460 nm and 535 nm. |
| In vitro | Fluorescence aging-related β-galactosidase (SA-β-Gal) assay [1]:
1.Cultivate cells at a density of 10^5 cells/mL.
2.Wash cells once with 200 μL PBS, then fix with 100 μL fixation solution (2% formaldehyde/0.2% glutaraldehyde in1. distilled water) at room temperature for 5 minutes.
3.Wash cells twice with 200 μL PBS, stain with 100 μL 33 μM C12FDG (PBS, pH 6.0) for 10 minutes, then stain with 200 μL Hoechst solution (1 μg/mL Hoechst 33342) in PBS (pH 6.0) for 10 minutes.
4.Image cells using a 20x objective and excitation filters of 360 nm (Hoechst 33342) and 480 nm (C12FDG), monitor cells using emission filters of 460 nm and 535 nm.
This protocol is provided for reference and should be modified according to the specific requirements of your experiment. |
| Storage | keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 30 mg/mL (35.13 mM), Sonication is recommended.
|
| Keywords | C12FDG |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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Recommended supplier
| Product name |
Price |
|
Suppliers |
Update time |
|
|
$1.00/1kg |
VIP7Y
|
Career Henan Chemical Co
|
2018-12-17 |
United States