Description of BIS-Tris from Watson Biotechnology Co., Ltd

Specifications(COA) of BIS-Tris CAS 6976-37-0 Buffering Agent

BIS-Tris (N,N-Bis(2-hydroxyethyl)-2-amino-2-(hydroxymethyl)-1,3-propanediol) is a buffering agent commonly used in biochemical and molecular biology applications. Here are some of its primary applications:

1. **pH Buffering in Biological Systems**: BIS-Tris is widely used as a buffering agent because it can maintain a stable pH in solutions, particularly within the pH range of 5.8 to 7.2. This makes it suitable for experiments involving proteins, enzymes, and other biological molecules, which often require a specific pH to maintain their structure and function.

2. **Electrophoresis**: In polyacrylamide gel electrophoresis (PAGE), BIS-Tris is used as a buffer in both the gel and running buffer. It helps maintain the pH and conductance necessary for the separation of proteins or nucleic acids based on size and charge.

3. **Protein Purification and Crystallization**: BIS-Tris is used in protein purification protocols, especially in affinity chromatography and ion exchange chromatography. It helps in maintaining the stability and solubility of proteins. Additionally, it is used in protein crystallization trials due to its buffering properties and minimal interference with protein crystallization.

4. **Enzyme Reactions**: BIS-Tris is used in various enzyme assays where maintaining a stable pH is crucial for optimal enzyme activity. It helps in studying enzyme kinetics and other biochemical properties of enzymes.

5. **Cell Culture Media**: Although less common than other buffers, BIS-Tris can be used in cell culture media formulations to maintain a stable pH environment, which is critical for cell growth and metabolism.

6. **General Laboratory Use**: As a versatile buffer, BIS-Tris can be used in a wide range of laboratory experiments, including DNA and RNA work, protein studies, and other biochemical assays.

Overall, BIS-Tris is favored in many biochemical applications due to its good buffering capacity, minimal interference with biological processes, and compatibility with a wide range of analytical techniques.

Founded in 2006 and headquartered in Wuhan, Hubei, China, Watson Biotechnology Co., Ltd is a leading manufacturing powerhouse specializing in the production of Active Pharmaceutical Ingredients (APIs) and Intermediates, Food and Feed Additives, Agricultural and Aquaculture chemicals, and Fine Chemicals. As a dedicated factory, our operations are driven by a commitment to excellence and innovation, ensuring that our products meet the highest standards of quality and compliance.

With a robust team of over 100 skilled professionals, Watson Biotechnology has established itself as a trusted name in the industry. Our products are meticulously crafted to align with international standards such as USP (United States Pharmacopeia), EP (European Pharmacopoeia), and BP (British Pharmacopoeia), guaranteeing reliability and efficacy.

At Watson Biotechnology, we pride ourselves on our cutting-edge facilities and state-of-the-art technology, which enable us to deliver superior products that cater to a diverse range of needs across various sectors. Our dedication to continuous improvement and customer satisfaction drives us to maintain stringent quality control processes and foster an environment of innovation and growth.

Watson Biotechnology Co., Ltd is a professional manufacturer which provide BIS-Tris with good quality and best price, welcome to contact us to get COA/TDS/MSDS of BIS-Tris.