Product Details
| Product Name:
Bioymifi |
CAS No.:
1420071-30-2 |
| Purity:
99.79% |
Supply Ability:
10g |
| Release date:
2024/11/17 |
Product Introduction
Bioactivity
| 名稱 | Bioymifi |
| 描述 | Bioymifi (DR5 Activator) is an effective activator of the TRAIL receptor DR5, binding to the extracellular domain (ECD) of DR5 with a Kd of 1.2 μM. Bioymifi can serve as a novel mimetic of TNF-related apoptosis-induced ligand (TRAIL), inducing DR5 aggregation as a sole inducer, and stimulating cell apoptosis. |
| 細(xì)胞實(shí)驗(yàn) | Dose-response curves of bioymifi and A2C2 in T98 g cells are plotted as a function of A2C2 or bioymifi concentration. Human glioblastoma (T98 g) cells are treated with various concentrations of A2C2 or bioymifi alone or in combination with 1 μM Smac mimetic (SM) for 48 h. The corresponding cell survival is normalized to the treatment without A2C2 or bioymifi.(Only for Reference) |
| 激酶實(shí)驗(yàn) | Solubilized membranes from 3T3 Flk-1 cells are added to polystyrene ELISA plates that has been precoated with a monoclonal antibody that recognizes Flk-1. After an overnight incubation with lysate at 4°C, serial dilutions of SU5416 are added to the immunolocalized receptor. To induce autophosphorylation of the receptor, various concentrations of ATP are added to the ELISA plate wells containing serially diluted solutions of SU5416. The autophosphorylation is allowed to proceed for 60 min at room temperature and then stopped with EDTA. The amount of phosphotyrosine present on the Flk-1 receptors in the individual wells is determined by incubating the immunolocalized receptor with a biotinylated monoclonal antibody directed against phosphotyrosine. After removal of the unbound anti-phosphotyrosine antibody, avidin-conjugated horseradish peroxidase H is added to the wells. A stabilized form of 3,3′,5,5′-tetramethyl benzidine dihydrochloride and Water2 is added to the wells. The color readout of the assay is allowed to develop for 30 min, and the reaction is stopped with H2SO4. Parallel biochemical kinase assays are performed to measure autophosphorylation on EGFR and fibroblast growth factor receptor[1]. |
| 體外活性 | METHODS: Erythrocytes were treated with Bioymifi (10-100 μM) for 24 h. The hemolytic and bactericidal properties and potential molecular mechanisms of Bioymifi were characterized by flow cytometry.
RESULTS: Bioymifi exerted a dose-responsive, calcium-independent hemolytic effect, reduced erythrocyte hemoglobin, significantly increased membrane-bound protein V, Fluo4, and DCF-positive cells, as well as a dual effect on forward and lateral light scatter. [1]
METHODS: Neonatal rat ventricular myocytes (nrvm) were treated with Bioymifi (10 μM) for 24 h, and the expression levels of target proteins were detected by Western Blot.
RESULTS: The expression of DR5 in Bioymifi-treated nrvm was higher than that of NRVF.[2] |
| 體內(nèi)活性 | METHODS: To investigate the role of DR5 activation in the heart, Bioymifi (5 mg/kg/d) micro-osmotic pump was administered to C57BL/6 mice once daily for two weeks.
RESULTS: Administration of Bioymifi resulted in hypertrophy at the level of cardiac organs and cardiomyocytes, but did not affect cardiomyocyte death or cardiac fibrosis. [2] |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 7.5 mg/mL (15.17 mM), Sonication is recommended.
|
| 關(guān)鍵字 | cancer | TNF Receptor | Inhibitor | TNFR | extracellular | inhibit | caspase-8 | Tumor Necrosis Factor Receptor | aggregation | domain | Apoptosis | Bioymifi | cells |
| 相關(guān)產(chǎn)品 | Stavudine | 5-Fluorouracil | Acetylcysteine | Kaempferol | Myricetin | Sodium 4-phenylbutyrate | L-Ascorbic acid | Dextran sulfate sodium salt (MW 4500-5500) | Metronidazole | Sorafenib | Tributyrin | Glucosamine |
| 相關(guān)庫 | 細(xì)胞凋亡化合物庫 | 經(jīng)典已知活性庫 | 細(xì)胞因子抑制劑庫 | HIF-1化合物庫 | NO PAINS 化合物庫 | 腫瘤免疫治療小分子化合物庫 | 已知活性化合物庫 | 共價抑制劑庫 | 抗癌化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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Recommended supplier
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$1.00/1KG |
VIP7Y
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Career Henan Chemical Co
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2019-09-05 |
United States