7-amino-4-methylcoumarin (Coumarin 120) is used as a fluorescent probe for many serine proteases.

IUPAC Name: 7-Amino-4-methylchromen-2-one 
Molecular Weight: 175.18396 g/mol
Molecular Formula: C₁₀H₉NO₂ 
Density: 1.275 g/cm³ 
Melting Point: 223-226 °C(lit.)
Boiling Point: 378.3 °C at 760 mmHg 
Flash Point: 216.9 °C
Index of Refraction: 1.627
Molar Refractivity: 48.7 cm³
Molar Volume: 137.3 cm³
Polarizability: 19.3×10^-24 cm³
Surface Tension: 52.5 dyne/cm 
Enthalpy of Vaporization: 62.61 kJ/mol
Vapour Pressure: 6.37E-06 mmHg at 25 °C 
Storage Temp.: 2-8 °C
Sensitive: light sensitive
XLogP3: 1.6
H-Bond Donor: 1
H-Bond Acceptor: 3
Tautomer Count: 7
Exact Mass: 175.063329
MonoIsotopic Mass: 175.063329
Topological Polar Surface Area: 52.3

Density: 1.275g /cm3

Boiling point: 378.3oC at 760 mmHg

Melting point: 223-226 °C(lit.)

Flash point: 216.9oC

Refractive index: 1.627

Stability: Stable under normal temperatures and pressures.

Storage conditions: 2-8°C

Vapor pressure: 6.37E-06mmHg at 25°C

Application:

Used as a laser dye. Fluorescent labeling reagent for trace enzyme detection. For high performance liquid chromatography labeling. Fluorescent labeling reagent for enzyme trace determination; Suitable for use as laser dye; It is used as a substrate for the analysis of monosulfated disaccharides and to form a co-substrate with 6-aza2chemicalbook thiothymine for the analysis of sulfated neutral and salivated trisugars and tetrasaccharides; Reagents for the preparation of novel AMC-based fluorescent substrates for cystine aminopeptidase (and other hydrolases); It is used as a reference compound in enzyme identification.