(-)-Blebbistatin NEW
| Price | $35 | $57 | $97 |
| Package | 2mg | 5mg | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: (-)-Blebbistatin | CAS No.: 856925-71-8 |
| Purity: 99.59% | Supply Ability: 10g |
| Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | (-)-Blebbistatin |
| Description | (-)-Blebbistatin ((S)-(-)-Blebbistatin) is an S enantiomer of blebbistatin. It is a potent and selective myosin II inhibitor with IC50 ranging from 0.5 to 5 μM. |
| Cell Research | Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3]. |
| Kinase Assay | Inhibition of Cox Activity: Inhibition of Cox-1 activity is measured by monitoring oxygen consumption during the conversion of arachidonic acid to PGs using a Clark-type O2-electrode. The reaction mixture contains ～0.2 units Cox-1 in 100 μL of microsome fraction derived from ram seminal vesicles as a crude source of Cox-1 (specific activity 0.2–1 units/mg protein) or 0.23 units of recombinant human Cox-2 (specific activity 43 units/mg protein). For calculation, the rate of O2 consumption is compared with a DMSO control (100% activity). Piroxicam, a nonsteroidal anti-inflammatory drug, is used as positive inhibitory substance for Cox-1 activity with an IC50 of 0.35 ± 0.05 μM (n = 2). Alternatively, nimesulide, a Cox-2 specific inhibitor, inhibits Cox-2 activity by 52 ± 5.7% (n = 2) at a concentration of 50 μM. |
| In vitro | METHODS: Mouse hepatic stellate cell HSCs were treated with (-)-Blebbistatin (12.5-50 μM) for 2 h and cell morphology was observed using microscopy. RESULTS: When treated with (-)-Blebbistatin for 2 h, the cells became smaller, acquired more dendritic morphology, and had fewer stress fibers and lamellipods. [1] METHODS: Porcine TM cells were treated with (-)-Blebbistatin (25-100 μM) for 2 h. The expression levels of target proteins were detected using Western Blot. RESULTS: There was no difference in MLC phosphorylation status between control and drug-treated samples. [2] |
| In vivo | Blebbistatin completely relaxes both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction in a dose-dependent manner. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4]. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 55 mg/mL (188.14 mM) 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2.92 mg/mL (9.99 mM), Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. |
| Keywords | inhibit | Blebbistatin | Myosin | Inhibitor | ( ) Blebbistatin | ()Blebbistatin |
| Inhibitors Related | Tirofiban hydrochloride monohydrate | Sodium oleate | Fenbufen | trans-Aconitic acid | Phlorizin | Chlorpropamide | Vonoprazan fumarate | Esomeprazole Magnesium | Revaprazan hydrochloride | Oleic acid | Esomeprazole Sodium | Trichlormethiazide |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $1520.00/25mg |
VIP1Y
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TargetMol Chemicals Inc.
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2024-10-23 |
United States- Since: 2011-01-07
- Address: 36?Washington?Street, Wellesley?Hills
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