ML-9 FREE BASE
| 中文名稱 | ML-9 FREE BASE |
|---|---|
| 中文同義詞 | 化合物 T16103;化合物 ML-9 FREE BASE |
| 英文名稱 | ML-9 |
| 英文同義詞 | ML-9 Free Base;ML9 Free Base,ML 9 Free Base;1H-1,4-Diazepine, 1-[(5-chloro-1-naphthalenyl)sulfonyl]hexahydro- |
| CAS號(hào) | 110448-31-2 |
| 分子式 | C15H17ClN2O2S |
| 分子量 | 324.83 |
| EINECS號(hào) | |
| 相關(guān)類別 | |
| Mol文件 | 110448-31-2.mol |
| 結(jié)構(gòu)式 |  |
ML-9 FREE BASE 性質(zhì)
| 沸點(diǎn) | 508.8±60.0 °C(Predicted) |
|---|---|
| 密度 | 1.327±0.06 g/cm3(Predicted) |
| 酸度系數(shù)(pKa) | 9.74±0.20(Predicted) |
ML-9 (Free Base) 是 Akt 激酶的選擇性強(qiáng)效抑制劑，可抑制肌球蛋白輕鏈激酶 (MLCK) 和基質(zhì)相互作用分子 1 (STIM1) 的活性。 ML-9 (Free Base) 抑制 MLCK，PKA 和 PKC 活性，Ki 值分別為 4、32 和 54 μM。 ML-9 通過刺激自噬小體的形成并抑制其降解來(lái)誘導(dǎo)自噬 (autophagy)。
ML9 (Free Base) (0-100?μM; 0-24?hours) has no reduction in cardiomyocyte viability, 50-100?μM significantly induces cell death. ML9 (Free Base) (50?μM; 1-4?hours) significantly increases cleaved?caspase-3?levels, decreased STIM1 protein levels by about 42%.
Cell Viability Assay
| Cell Line: | Neonatal rat ventricular?myocytes?(NRVM)?cells |
| Concentration: | 0, 10, 50 and 100?μM |
| Incubation Time: | 0, 1, 4, 8 and 24?hours |
| Result: | Decreased cell viability at 50–100?μM concentration. |
Apoptosis Analysis
| Cell Line: | Neonatal rat ventricular?myocytes?(NRVM)?cells |
| Concentration: | 50?μM |
| Incubation Time: | 0, 1, 4, 8 hours |
| Result: | Induced cardiomyocyte death through?necrosis?and apoptosis. |