ANTI-SF1 抗體性質(zhì)、用途與生產(chǎn)工藝

描述

兔單克隆抗體[EPR9105(B)] to Tat-SF1

宿主

Rabbit

種屬反應(yīng)性

與反應(yīng): Rat, Human

制備

Produced in rabbits immunized with E. coli-derived Human HTATSF1 fragment, and purified by antigen affinity chromatography.

驗證的應(yīng)用

WB,IHC-P,ICC/IF,IP

品牌示例

All lanes : Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 86 kDa



Western blot - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

背景

This gene encodes a nuclear pre-mRNA splicing factor. The encoded proteinspecifically recognizes the intron branch point sequence at the 3' splice site,together with the large subunit of U2 auxiliary factor (U2AF), and is required for theearly stages of spliceosome assembly. It also plays a role in nuclear pre-mRNAretention and transcriptional repression. The encoded protein contains an N-terminalU2AF ligand motif, a central hnRNP K homology motif and quaking 2 region whichbind a key branch-site adenosine within the branch point sequence, a zinc knucklesdomain, and a C-terminal proline-rich domain. Alternative splicing results in multipletranscript variants.

檢測原理

雙抗體夾心法測定標(biāo)本中SF1水平。用純化的SF1抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入SF1，再與HRP標(biāo)記的SF1抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的SF1呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中SF1濃度。

解旋酶

解旋酶是最大的一類酶，幾乎所有涉及DNA或RNA的生物過程都使用一種或多種解旋酶。很多疾病與解旋酶的功能缺陷以及蛋白質(zhì)表達(dá)失控有關(guān)，包括癌癥、發(fā)育缺陷和神經(jīng)退行性疾病。分為環(huán)狀為主的六面體結(jié)構(gòu)，由SF3至6組成，未形成環(huán)的酶由SF1和2組成。以前基于序列、結(jié)構(gòu)和機制特征分類，但這些分類都沒有擴展到SF1和SF2的所有成員，本文基于不同的蛋白質(zhì)家族分類。
超家族SF1和2的解旋酶幾乎參與RNA和DNA代謝的各個方面，有相似的催化核心結(jié)構(gòu)。基于蛋白質(zhì)家族的分類，具有典型的序列、結(jié)構(gòu)和機制特征。這個分類補充和擴展了現(xiàn)有的SF1和SF2解旋酶分類，并突出蛋白質(zhì)的主要結(jié)構(gòu)和功能。SF1和SF2解旋酶的標(biāo)志是一個保守的解旋酶核心，由兩個相似的蛋白結(jié)構(gòu)域組成，類似于重組蛋白RecA的折疊，含有特征序列基序，在此基礎(chǔ)上完成了解旋酶在SFS中的原始細(xì)分。

ANTI-SF1 抗體 上下游產(chǎn)品信息

上游原料

下游產(chǎn)品

ANTI-SF1 抗體 生產(chǎn)廠家

 

ANTI-SF1 抗體 相關(guān)搜索:

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• Anti-FTZ-F1beta
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• Anti-B1F
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• Anti-FTZF1
• Anti-FTZ1
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