天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

返回ChemicalBook首頁(yè)>CAS數(shù)據(jù)庫(kù)列表>98849-88-8

98849-88-8

中文名稱 FLAG PEPTIDE
英文名稱 FLAG peptide
CAS 98849-88-8
分子式 C41H60N10O20
分子量 1012.97
MOL 文件 98849-88-8.mol
更新日期 2024/12/22 20:23:43
98849-88-8 結(jié)構(gòu)式 98849-88-8 結(jié)構(gòu)式

基本信息

中文別名
DDDDK肽
L-LYSINE, L-Α-ASPARTYL-L-TYROSYL-L-LYSYL-L-Α-ASPARTYL-L-Α-ASPARTYL-L-Α-ASPARTYL-L-Α-ASPARTYL-
英文別名
FLAG®
FLAG tag Peptide
FLAG PEPTIDE, >98%
FLAG Tag Peptide,F(xiàn)LAG epitope
FLAG PEPTIDE|DYKDDDDK|ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
FLAG peptide,DYKDDDDK,Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, >98%
(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-amino-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]hexanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]hexanoic acid
所屬類別
生物化工:多肽

物理化學(xué)性質(zhì)

密度1.470±0.06 g/cm3 (20 ºC 760 Torr)
儲(chǔ)存條件Keep in dark place,Sealed in dry,Store in freezer, under -20°C
溶解度≥50.65 mg/mL in DMSO; ≥210.6 mg/mL in H2O; ≥34.03 mg/mL in EtOH
形態(tài)凍干粉
顏色White to off-white
序列H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH
InChIKeyXZWYTXMRWQJBGX-OVIGALBNNA-N

常見問題列表

生物活性
FLAG peptide是一種八個(gè)氨基酸組成的多肽 (Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys),具有腸激酶切割位點(diǎn)。FLAG peptide用于抗體介導(dǎo)的重組蛋白的識(shí)別和純化。
體外研究

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca 2+ . Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca 2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.

"98849-88-8" 相關(guān)產(chǎn)品信息