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基本信息
胃粘膜素
發(fā)胃膜素
胃粘液素
粘液素III
粘液素 II
胃膜素(豬)
粘蛋白,來源于豬粘膜消化
胃粘膜素、粘液素 II、胃膜素
粘蛋白(胃粘膜素、粘液素、胃膜素)
Gastron
MUCIN TYPE I
MUCIN TYPE II
MUCIN GASTRIC
Gastric Mucin
MUCIN TYPE I-S
MUCIN TYPE III
Mutin(gastric)
Mucins, gastric
物理化學(xué)性質(zhì)
制備方法
消化 將胃漿膜絞碎,置于耐酸夾層鍋。攪拌下,按1800ml HCl:100kg胃膜比例,于pH 3-3.5,40-45℃消化4h,至消化液半透明止。雙層紗布過濾除去雜質(zhì),用50%堿調(diào)pH 4-5得消化液。
胃黏膜[絞碎]→胃黏膜漿[HCl, NaOH]→[pH3-3.5, 40-45℃]消化液
醇沉淀 消化液攪拌下加入80%以上乙醇,含醇量達70%左右,0-5℃靜置12h,收集沉淀,壓干。按濕重加60%-70%乙醇2-3倍,混合均勻,洗滌1-2次,沉淀壓干,搓碎,在70℃左右干燥,得粗制胃膜素。
消化液[乙醇]→[0-5℃, 12h]粗制胃膜素
脫脂干燥 將粗制胃膜素粉裝袋,用乙醚脫脂,揮去乙醚在50℃烘干。粉碎、過80目篩得胃膜素。
粗制胃膜素[乙醚]→脫脂胃膜素[粉碎]→胃膜素成品
方法二、丙酮分級沉淀法
消化 按每1000g胃黏膜加入18ml HCl,在pH 2.5-3, 45℃攪拌3-4h,使消化。雙層紗布過濾得消化液,5℃以下冷庫保存。
胃黏膜漿[HCl]→[pH2.5-3, 45℃, 3-4h]消化液
沉淀胃膜素 次日取消化液,常溫攪拌緩加5℃以下的丙酮,使溶液相對密度達0.94-0.96,析出胃膜素,雙層紗布過濾,得粗晶,70%乙醇洗滌兩次,70℃干燥,乙醚脫脂兩次,烘干,粉碎,得胃膜素精品。
沉淀胃蛋白酶 取沉淀胃膜素后的母液,攪拌補加5℃以下的丙酮,使相對密度達0.89-0.91, 靜置4h使沉淀,虹吸去上清液回收丙酮。收集沉淀,70℃干燥,粉碎,得胃蛋白酶成品。
母液[冷丙酮]→[相對密度0.89-0.91]胃蛋白酶[干燥]→胃蛋白酶成品。
取胃黏膜絞碎,用鹽酸調(diào)pH 3,40℃消化得消化液,0-5℃下,乙醇沉淀,過濾得粗制胃膜素。
胃黏膜→胃黏膜漿[HCl]→[pH3, 40℃]消化液[乙醇]→[0-5℃]粗制胃膜素
將上述粗制胃膜素,乙醚脫脂后,干燥得脫脂胃膜素,粉碎即得成品。
粗制胃膜素[乙醚]→脫脂胃膜素→成品
胃膜素膠囊的制備 取胃膜素精品,按膠囊劑要求制成胃膜素膠囊。
胃膜素精品→胃膜素膠囊
方法二、丙酮分級沉淀法
取胃黏膜絞碎成胃黏膜漿,鹽酸調(diào)節(jié)pH 2.5,45℃下消化,進一步將消化液,冷丙酮沉淀,過濾,得胃膜素粗品。
胃黏膜→胃黏膜漿[HCl]→[pH2.5, 45℃]消化液[冷丙酮]胃膜素粗品
將上述胃膜素粗品,經(jīng)乙醇、乙醚洗滌、脫脂、干燥后,得胃膜素精品。母液經(jīng)冷丙酮沉淀,過濾,得胃蛋白酶,干燥即得胃蛋白酶成品。
胃膜素粗品[乙醇,乙醚]→[洗滌,脫脂,干燥]胃膜素精品
母液[冷丙酮]→[沉淀胃蛋白酶]胃蛋白酶[干燥]→胃蛋白酶成品。
常見問題列表
Gastric mucin may be integrally involved in the mechanism of gastric mucosal injury caused by Helicobacter pylori leading to gastritis, peptic ulceration, and possibly gastric cancer. Gastric mucins are classified into two types based on their histochemical properties. The first is a surface mucous cell-type mucin, secreted from the surface mucous cells. The second is found in deeper portions of the mucosa and is secreted by gland mucous cells, including mucous neck cells, cardiac gland cells, and pyloric gland cells. The unique O-glycans in gastric mucin appears to function as a natural antibiotic, protecting the host from H. pylori infection. Gastric mucin may provide protection to the surface epithelium gastrointestinal tract by scavenging oxidants produced within the lumen; however, it does so at the expense of its viscoelastic properties. Both native and pronase-treated mucin effectively scavenge hydroxyl radical and that the scavenging properties are not significantly different. The effective concentration of mucin required for a 50% reduction in malondialdehyde production is 10 mg/mL for both native and pronase-treated mucin.