天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

返回ChemicalBook首頁>CAS數(shù)據(jù)庫列表>70132-51-3

70132-51-3

中文名稱 PIMONIDAZOLE
英文名稱 pimonidazole
CAS 70132-51-3
分子式 C11H19ClN4O3
分子量 290.747
MOL 文件 70132-51-3.mol
70132-51-3 結(jié)構(gòu)式 70132-51-3 結(jié)構(gòu)式

基本信息

中文別名
化合物 T16536
化合物PIMONIDAZOLE HYDROCHLORIDE
英文別名
PIMONIDAZOLE HCL

物理化學(xué)性質(zhì)

儲存條件-20°C儲存
溶解度Water: 5 mg/mL (17.20 mM; ultrasonic and warming and heat to 80°C)
形態(tài)Solid
顏色White to yellow

常見問題列表

生物活性
Pimonidazole 是一種缺氧標(biāo)記物,用于瘤內(nèi)缺氧和細(xì)胞增殖的補(bǔ)充性研究。Pimonidazole 通過與大分子的共價(jià)結(jié)合或通過被還原形成還原性代謝物后在缺氧細(xì)胞中積累,可用于定性和定量評估腫瘤缺氧。
體外研究

Pimonidazole, the exogenous hypoxia marker, is a 2-nitroimidazole compound, which forms covalent bonds with cellular macromolecules at oxygen levels below 1.3%.
Detection : Hypoxic cells were recognized by immunohistochemical detection of pimonidazole using a mouse monoclonal antibody. Cell proliferation was detected with a commercially available monoclonal antibody for proliferating cell nuclear antigen (PCNA). Assessment of hypoxia and cell proliferation was made qualitatively with light microscopy and quantitatively using point counting and image analysis software methods.
The pimonidazole-protein adducts were then detected by immunofluorescence Cells (90% confluent) are treated with pimonidazole hydrochloride (10-100 μM), diluted in complete media or PBS, and placed in hypoxia or normoxia for 2-4 hours.Then, cells are washed 4 times in HBSS, fixed for 10 min with 10% neutral buffered formalin at room temperature, washed 3 times with tris buffered saline, and mounted with Prolong Gold with DAPI mounting media.To monitor the intrinsic hypoxic signal, the tissue is removed and the embryos incubated with 400 μM pimonidazole for 2 h before fixation. Immunostaining is performed using antibodies against pimonidazole-protein adducts (Hypoxyprobe).

體內(nèi)研究

Pimonidazole is a 2-nitroimidazole that is reductively activated specifically in hypoxic cells and forms stable adducts with thiol groups in proteins, peptides, and amino acids. Additionally, the amount of pimonidazole that is detected is directly proportional to the level of hypoxia within tumors in vivo. The following protocol is provided for this useful tool to interrogate the levels of hypoxia in vivo . 1.Pimonidazole hydrochloride is suspended at a concentration of 30 mg/ml in 0.9% sterile saline or PBS. 2.Multiple mice are injected intravenously (tail vein) with 60 mg/kg of the pimonidazole solution. 3.Let circulate?in vivo?for 90 min before the mice are euthanazied with isoflurane or carbon dioxide (CO2). Note: For animal euthanasia: In compliance with proper IACUC protocols! 4. Mice are sacrificed and organs are then removed, weighed, snap-frozen, and stored in plastic scintillation vials at -80 °C. 5. The pimonidazole stain can work on paraffin-embedded tissue, frozen tissue, and cell lines. Organs are embedded in OCT, placed in a cryostat, and cut into 10 μm-thick sections. Sections are placed on microscope slides for staining.

"70132-51-3" 相關(guān)產(chǎn)品信息