494753-69-4
494753-69-4 結(jié)構(gòu)式
基本信息
Ginsenoside RK2 USP/EP/BP
Ginsenoside Rk1 (Rk1:Rz1=5:2)
3beta,12beta-Dihydroxydammar-20(21),24-diene-3-O-beta-D-glucopyranosyl(1→
3β,12β-dihydroxydammar-20(21),24-diene-3-O-β-D-glucopyranosyl(1->2)-β-D-glucopyranoside
β-D-Glucopyranoside, (3β,12β)-12-hydroxydammara-20,24-dien-3-yl 2-O-β-D-glucopyranosyl-
3beta,12beta-Dihydroxydammar-20(21),24-diene-3-O-beta-D-glucopyranosyl(1-2)-beta-D-glucopyranoside
物理化學性質(zhì)
應用領(lǐng)域
藥理藥效:抗癌、提高免疫功能、調(diào)節(jié)神經(jīng)系統(tǒng)。
常見問題列表
Rk2一般通過水解人參皂苷二醇組獲得，但水解成分復雜，不利于分離并準確鑒定結(jié)構(gòu)。本文通過酶法水解高純度的人參皂苷Rk2，水解產(chǎn)物通過高分辨質(zhì)譜和核磁確證。
Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation.
RT-PCR
| Cell Line: | RAW264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 6 hours |
| Result: | Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells. |
Western Blot Analysis
| Cell Line: | RAW264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 6 hours |
| Result: | Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells |
Cell Viability Assay
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 48 hours |
| Result: | Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner. |
Cell Cycle Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 24 hours |
| Result: | Induced G0/G1 phase arrest. |
Apoptosis Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis in MDA-MB-231 cells. |