325143-98-4
325143-98-4 結(jié)構(gòu)式
基本信息
生物素-二硫鍵-磺酸-NHS 酯
生物素-二硫鍵-磺酸-琥珀酰亞胺酯
BIOTIN-SS-SULFO-OSU
磺酸基-N-琥珀酰亞胺基酯-雙硫鍵-生物素
1-[3-[[2-[[5-[(3AS,4S,6AR)-六氫-2-氧代-1H-噻吩并[3,4-D]咪唑-4-基]-1-氧代戊基]氨基]乙基]二硫基]-1-氧代丙氧基]-2,5-二氧代-3-吡咯烷磺酸鈉
Biotin-SS-Sulfo-NHS
NHS-SS-BIOTIN SodiuM Salt
Biotin-SS-Sulfo-NHS ester
Sulfo-NHS-SS-Biotin sodium
EZ-link sulfo-NHS-SS-Biotin
SulfosucciniMidyl 3-[[2-(BiotinaMido)ethyl] dithio]propionate SodiuM Salt
3-[[2-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]iMidazol-4-yl]-1-oxopentyl]aMino]ethyl]dithio]-propanoic Acid 2,5-dioxo-3-sulfo-1-pyrrolidinyl Ester, SodiuM Salt
1-[3-[[2-[[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]ethyl]dithio]-1-oxopropoxy]-2,5-dioxo-3-pyrrolidinesulfonic acid monosodium salt
常見問題列表
IC50: cell-surface protein
Sulfo-NHS-SS-biotin is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes. Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides. Sulfo-NHS-SS-biotin has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns. Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail ( HY-K0010 ). Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer.