常見問題列表
生物活性
BRD5631 是一種自噬 (autophagy) 增強劑，通過與 mTOR 無關的通路來增強自噬。BRD5631 能夠影響先前與自噬相關的幾種細胞疾病表型，包括蛋白聚集、細胞存活、細菌復制和炎性因子的產生。靶點
Autophagy
體外研究
BRD5631 (10 μM, 48 hours) leads to increased levels of LC3-II in HeLa cells. BRD5631 stimulates formation of new autophagosomes, which is consistent with its ability to increase numbers of autolysosomes in the mCherry-GFP-LC3 assay.
BRD5631 does not enhance autophagy by directly inhibiting mTOR or the mTOR signaling pathway. BRD5631 suppressed IL-1β secretion in an autophagy-dependent manner.
Cell Viability Assay
| Cell Line: | HeLa cells. |
| Concentration: | 10 μM. |
| Incubation Time: | 48 hours. |
| Result: | Led to increased levels of LC3-II. |