210892-23-2
210892-23-2 結(jié)構(gòu)式
基本信息
CY5.5熒光染料
CY5雙酸-N-羥基琥珀酰亞胺酯
2-[5-[3-(5-羧基戊基)-1,3-二氫-1,1-二甲基-6,8-二磺基-2H-苯并[E]吲哚-2-亞基]-1,3-戊二烯基]-3-乙基-1,1-二甲基-6,8-二磺基-1H-苯并[E]吲哚鎓內(nèi)
2-[5-[3-(5-羧基戊基)-1,3-二氫-1,1-二甲基-6,8-二磺基-2H-苯并[E]吲哚-2-亞基]-1,3-戊二烯基]-3-乙基-1,1-二甲基-6,8-二磺基-1H-苯并[E]吲哚鎓內(nèi)鹽
Cy5.5, >97%
Sulfo-Cy5.5
Cy5 diacid, SE
Sulfo-Cyanine5.5
Bis Benzimide Hoechst NO33258
Bis Benzlimide Hoechst NO33342
Sulfo Cy5.5 Carboxylic acids(ethyl)
2-((1E,3E,5E)-5-(3-(5-Carboxypentyl)-1,1-diMethyl-1H-benzo[e]indol-2(3H)-ylidene)penta-1,3-dienyl)-3-ethyl-1,1-diMethyl-1H-benzo[e]indoliuM chloride
2-(5-(3-(5-carboxypentyl)-1,1-dimethyl-6,8-disulfo-1,3-dihydro-2H-benzo[e]indol-2-ylidene)penta-1,3-dien-1-yl)-3-ethyl-1,1-dimethyl-8-sulfo-1H-benzo[e]indol-3-ium-6-sulfonate
常見問題列表
Cy5.5是氰基染料的一種，氰基的定義是“合成染料，其通式為R2N[CH=CH]nCH=N+R2，分解為R2N+=CH[CH=CH]nNR2 (N是一個小數(shù))，其中氮和部分共軛鏈通常構(gòu)成雜環(huán)系統(tǒng)的一部分，如咪唑、吡啶、吡role、喹啉和噻唑。”氰基染料用于工業(yè)生物技術(shù)(標(biāo)記、分析、生物醫(yī)學(xué)成像)。
因為氰酸能產(chǎn)生更亮更穩(wěn)定的熒光，所以可以很好地取代傳統(tǒng)的染料，如熒光素和羅丹明。Cy3和Cy5是最常用的，通常用于兩種顏色的檢測。Cy3發(fā)出綠黃色熒光(約550 nm激發(fā)，約570 nm發(fā)射)，而Cy5在紅色區(qū)域發(fā)出熒光(約650 nm激發(fā)，約670 nm發(fā)射)。
Cy5成為流行的紅色熒光染料替代品是由于其高消光系數(shù)(在凝膠電泳可以肉眼發(fā)現(xiàn)1 nanomol)。Cy5.5是一種近紅外(IR)熒光發(fā)射染料(激發(fā)/發(fā)射最大值678/694 nm)。
熒光染料Cy5.5 (Sulfo-Cyanine5.5) 是用于標(biāo)記生物分子的近紅外熒光染料 (Ex=673 nm, Em=707 nm)，如肽，蛋白質(zhì)和寡核苷酸。
熒光染料主要能吸收可見光波和紫外光波，并能將紫外光轉(zhuǎn)變?yōu)椴ㄩL較長的可見光波而反射出來。例如，酸性曙紅、熒光黃、紅汞以及某些分散染料等。它們大多是含有苯環(huán)或雜環(huán)并帶有共軛雙鍵的化合物。
熒光染料具有閃光的鮮艷色彩，除用于纖維織物印染外，還可用作某些特種標(biāo)志(如暗處符號)及軍事追蹤等。熒光染料中含有能與核酸、碳水化合物和肽共軛連接的基團，該共軛物一般情況下穩(wěn)定性好，在紫外可見光的照射下，相比于其他的熒光物質(zhì)或顏色標(biāo)記物具有更優(yōu)異的溶劑變色效應(yīng)。它還可用于滲漏污水系統(tǒng)、水和工業(yè)的污染物、連接系統(tǒng)、測量發(fā)電廠排出的液體、洗手間的滲漏、非法的連接污水管監(jiān)察，研究流量和繪圖、分析腐敗的系統(tǒng)。
Cy5.5 contains a free amine group which can be conjugated with a variety of functionalities, including NHS esters, and epoxides. The excitation and emission maximum are 678 and 694 nm, respectively. Cy5.5 has been applied in various fluorescent based experiments. Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. Cy5.5 is cost-effective and its labeling chemistries are easy to perform, making it suitable for potential anti-cancer drug development.
Cy5.5-labeled factor VIIa is developed for imagining cancer. Cy5.5 labeled with these targeting proteins specifically localize to the tumor xenografts for at least 14 days but unconjugated Cy5.5 does not localize to any xenografts or organs. This method of imaging anti-tissue factor in the tumor VECs may be useful in detecting primary tumors and metastases as well as monitoring in vivo therapeutic responses. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin (Cy5.5-Lf-MPNA nanogels) are developed as a promising contrast agent for preoperative MRI and intraoperative fluorescence imaging of glioma.