2097938-51-5
中文名稱
METHYL 2-(1-(7H-PYRROLO[2,3-D]PYRIMIDIN-4-YL)PIPERIDIN-4-YL)-1H-BENZO[D]IMIDAZOLE-6-CARBOXYLATE
英文名稱
1H-Benzimidazole-6-carboxylic acid, 2-[1-(5-chloro-7H-pyrrolo[2,3-d]pyrimidin-4-yl)-4-piperidinyl]-, methyl ester
CAS
2097938-51-5
分子式
C20H19ClN6O2
分子量
410.86
MOL 文件
2097938-51-5.mol
更新日期
2023/03/20 15:41:19
2097938-51-5 結(jié)構(gòu)式
基本信息
中文別名
化合物R-10015 英文別名
methyl 2-(1-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperidin-4-yl)-1H-benzo[d]imidazole-6-carboxylateMethyl 2-(1-(5-chloro-7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperidin-4-yl)-1H-benzo[d]imidazole-5-carboxylate
1H-Benzimidazole-6-carboxylic acid, 2-[1-(5-chloro-7H-pyrrolo[2,3-d]pyrimidin-4-yl)-4-piperidinyl]-, methyl ester
物理化學(xué)性質(zhì)
密度1.465±0.06 g/cm3(Predicted)
儲(chǔ)存條件-20°C儲(chǔ)存
溶解度DMSO : 62.5 mg/mL (152.12 mM; Need ultrasonic)
酸度系數(shù)(pKa)10.68±0.10(Predicted)
形態(tài)Solid
顏色Off-white to pink
常見(jiàn)問(wèn)題列表
生物活性
R-10015 是一種有效的、具有選擇性的 LIM domain kinase (LIMK) 抑制劑，對(duì)于人LIMK1的IC50值為38 nM。R-10015 會(huì)結(jié)合到 ATP-binding pocket 上，并充當(dāng)HIV感染的廣譜抗病毒化合物。靶點(diǎn)
| Target | Value |
|
hLIMK1
(Cell-free assay) | 38 nM |
體外研究
R-10015 (100 μM; 0-4 hours) inhibits cofilin phosphorylation directly through blocking LIM kinase in CEM-SS T cells.
R-10015 inhibits HIV-1 DNA synthesis, nuclear migration, and virion release.
R-10015 inhibits multiple viruses, including Zaire ebolavirus (EBOV), Rift Valley fever virus (RVFV), Venezuelan equine encephalitis virus (VEEV), and herpes simplex virus 1 (HSV-1) .
Western Blot Analysis
| Cell Line: | CEM-SS T cells |
| Concentration: | 100 μM |
| Incubation Time: | 0 hour,0.5 hour,1 hour,2 hours,4 hours |
| Result: | Inhibited cofilin phosphorylation directly through blocking LIM kinase in CEM-SS T cells. |
體內(nèi)研究
R-10015 (10 mg/kg; i.p.) displays none indication of toxicity. The result suggests the possibility of short-term use of LIMK inhibitors to block viral infections.
| Animal Model: | 6-8 weeks female C3H/HeN mice |
| Dosage: | 10 mg/kg |
| Administration: | Intraperitoneal injection |
| Result: | Displayed none indication of toxicity. |