1895050-66-4
1895050-66-4 結(jié)構(gòu)式
物理化學(xué)性質(zhì)
密度1.41±0.1 g/cm3(Predicted)
儲存條件-20°C儲存
溶解度溶于二甲基亞砜
酸度系數(shù)(pKa)8.04±0.70(Predicted)
形態(tài)Solid
顏色Off-white to light yellow
常見問題列表
生物活性
6RK73 是一種共價不可逆的特異性 UCHL1 抑制劑，IC50 為 0.23 μM。6RK73 對 UCHL3 幾乎沒有抑制效果 (IC50=236 μM)。6RK73 特異性抑制乳腺癌中 UCHL1 的活性。靶點(diǎn)
IC50: 0.23 μM (UCHL1), 236 μM (UCHL3)
體外研究
6RK73 (5 μM; 1-3 hours) treatment displays strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels in MDA-MB-436 cells.
6RK73(5 μM; 24-48 hours) results migration significantly slower than the DMSO control group in MDA-MB-436 cells.
Cell Viability Assay
| Cell Line: | MDA-MB-436 cells |
| Concentration: | 5 μM |
| Incubation Time: | 24, 48 hours |
| Result: | Migrated significantly slower than the DMSO control group |
Western Blot Analysis
| Cell Line: | MDA-MB-436 cells |
| Concentration: | 5 μM |
| Incubation Time: | 1, 2, 3 hours |
| Result: | Displayed strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels. |
體內(nèi)研究
6RK73 displays a potent inhibition of breast cancer extravasation in zebrafish.