188532-26-5
188532-26-5 結(jié)構(gòu)式
基本信息
5-(3,5-二叔丁基-4-羥基芐基)噻唑烷-2,4-二酮
2,4-Thiazolidinedione, 5-[[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]-
物理化學(xué)性質(zhì)
常見問題列表
MitoNEET
NL-1 (10-100 μM; 72 hours; REH, REH/Ara-C and ALL cell lines) treatment reduces the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. NL-1 inhibits SUPB15, NALM6 with
IC
50
s of 29.48 μM, 94.26 μM, respectively. TOM1, BV173, NALM1 and JM1 all have similar
IC
50
values of around 60 μM for NL-1.
NL-1 (60 μM; 6 hours; REH, REH/Ara-C cell lines) treatment mediates autophagy, and inhibition of autophagy partially decreased NL-1-induced tumor cell death.
NL-1 pretreatment inhibits the chemotactic ability of both REH and REH/Ara-C cells to migrate towards multiple chemoattractants. The cells treated with NL1 shows a dose-dependent decrease in chemotaxis both in the REH and the REH/AraC cells.
Cell Proliferation Assay
| Cell Line: | REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines |
| Concentration: | 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, 100 μM |
| Incubation Time: | 72 hours |
| Result: | Reduced the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. |
Cell Autophagy Assay
| Cell Line: | REH, REH/Ara-C cell lines |
| Concentration: | 60 μM |
| Incubation Time: | 6 hours |
| Result: | Induced cell autophagy. |
NL-1 (10 mg/kg; intraperitoneal injection; daily; for 5 days; female NSG mice) treatment shows antileukemic activity in an in vivo mouse ALL model.
| Animal Model: | 10 female NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells |
| Dosage: | 10 mg/kg |
| Administration: | Intraperitoneal injection; daily; for 5 days |
| Result: | Showed antileukemic activity in an in vivo mouse ALL model. |