168649-23-8
中文名稱
5-(2-苯基)-吲哚-3-乙酸
英文名稱
1H-Indole-3-acetic acid, 5-phenyl-
CAS
168649-23-8
分子式
C16H13NO2
分子量
251.28
MOL 文件
168649-23-8.mol
更新日期
2024/12/22 21:55:00
168649-23-8 結(jié)構(gòu)式
基本信息
中文別名
化合物5-PH-IAA5-苯基-1H-吲哚-3-乙酸
5-(2-苯基)-吲哚-3-乙酸
英文別名
5-Ph-IAA5-(2-phenyl)-indole-3-acetic acid
1H-Indole-3-acetic acid, 5-phenyl-
所屬類別
醫(yī)藥中間體：原料藥中間體物理化學(xué)性質(zhì)
沸點(diǎn)521.3±35.0 °C(Predicted)
密度1.291±0.06 g/cm3(Predicted)
儲(chǔ)存條件-20°C儲(chǔ)存
溶解度DMSO: 125 mg/mL (497.45 mM)
酸度系數(shù)(pKa)4.47±0.30(Predicted)
形態(tài)固體
顏色White to off-white
常見(jiàn)問(wèn)題列表
生物活性
5-Ph-IAA 是 IAA 的衍生物。5-Ph-IAA 是一種配體與 OsTIR1 (F74G) 突變體一起建立了生長(zhǎng)素誘導(dǎo)的 degron 2 系統(tǒng) (AID2)。AID2 誘導(dǎo) mAID 融合蛋白的快速有效消耗，從而研究活細(xì)胞中的蛋白功能。AID2 可以抑制腫瘤。體外研究
HCT116 cells constitutively expressing OsTIR1(F74G) are exposed to 5-Ph-IAA (1 μM; 6 hours), leading to DHC1-mAC degradation, which means the AID2 system better than the original AID system in generating conditional mutant cell lines.
Western Blot Analysis
| Cell Line: | HCT116 cells |
| Concentration: | 1 μM |
| Incubation Time: | 6 hours |
| Result: | Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G). |
體內(nèi)研究
5-Ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system ,is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumour suppression.
| Animal Model: | Balb/c-nu female mice (7 weeks old; 16–20?g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells |
| Dosage: | 0 mg/kg, 1 mg/kg, 3 mg/kg and 10?mg/kg |
| Administration: | Intraperitoneally injection; every day; 7 days |
| Result: | Displayed significant tumour suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts. |