124689-65-2
中文名稱
CS-1374
CAS
124689-65-2
分子式
C35H43ClN2O8
分子量
655.18
124689-65-2 結(jié)構(gòu)式
基本信息
英文別名
CS-1374Cryptophycin
cryptophycin 1
Cyclo[(2R)-2-methyl-β-alanyl-(2S)-2-hydroxy-4-methylpentanoyl-(2E,5S,6S)-5-hydroxy-6-[(2R,3R)-3-phenyl-2-oxiranyl]-2-heptenoyl-3-chloro-O-methyl-D-tyrosyl]
常見問題列表
生物活性
Cryptophycin 1 是一種從 Nostoc sp. 中分離出來的有效的細胞毒性抗微管劑。Cryptophycin 1 可以誘導(dǎo)細胞凋亡，并具有抗腫瘤活性和出色的抗增殖能力。體外研究
Cryptophycin 1 (50 pM) rapidly causes morphological changes consistent with the induction of apoptosis in SKOV3 and MDA-MB-435 cells.
Cryptophycin 1 (50 pM; 12-24 h) causes an accumulation of MDA-MB-435 cells in G2/M.
Cryptophycin 1 (100 nM; 1-7 min) markedly reduces the rates and extents of microtubule shortening and growing events and dramatically increases the duration of phases of attenuation or pause.
Apoptosis Analysis
| Cell Line: | SKOV3 cells |
| Concentration: | 50 pM |
| Incubation Time: | 4 hours |
| Result: | Caused rapid cell shrinkage, loss of the normal elliptical spheroid shape and the formation of membrane blebs. |
Cell Cycle Analysis
| Cell Line: | MDA-MB-435 cells |
| Concentration: | 50 pM |
| Incubation Time: | 12, 18, 24 hours |
| Result: |
A doubling in the number of cells in G
2
/M at 12 hr.
Still a large number of cells (58%) in G 2 /M at 18 hr. |
體內(nèi)研究
Cryptophycin 1 (i.v.) exhibits anti-tumor activity in mice.