110448-31-2
110448-31-2 結(jié)構(gòu)式
常見(jiàn)問(wèn)題列表
生物活性
ML-9 (Free Base) 是 Akt 激酶的選擇性強(qiáng)效抑制劑，可抑制肌球蛋白輕鏈激酶 (MLCK) 和基質(zhì)相互作用分子 1 (STIM1) 的活性。 ML-9 (Free Base) 抑制 MLCK，PKA 和 PKC 活性，Ki 值分別為 4、32 和 54 μM。 ML-9 通過(guò)刺激自噬小體的形成并抑制其降解來(lái)誘導(dǎo)自噬 (autophagy)。體外研究
ML9 (Free Base) (0-100?μM; 0-24?hours) has no reduction in cardiomyocyte viability, 50-100?μM significantly induces cell death. ML9 (Free Base) (50?μM; 1-4?hours) significantly increases cleaved?caspase-3?levels, decreased STIM1 protein levels by about 42%.
Cell Viability Assay
| Cell Line: | Neonatal rat ventricular?myocytes?(NRVM)?cells |
| Concentration: | 0, 10, 50 and 100?μM |
| Incubation Time: | 0, 1, 4, 8 and 24?hours |
| Result: | Decreased cell viability at 50–100?μM concentration. |
Apoptosis Analysis
| Cell Line: | Neonatal rat ventricular?myocytes?(NRVM)?cells |
| Concentration: | 50?μM |
| Incubation Time: | 0, 1, 4, 8 hours |
| Result: | Induced cardiomyocyte death through?necrosis?and apoptosis. |