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ChemicalBook--->CAS DataBase List--->9014-08-8

9014-08-8

9014-08-8 Structure

9014-08-8 Structure
IdentificationBack Directory
[Name]

ENOLASE
[CAS]

9014-08-8
[Synonyms]

ENOLASE
EC 4.2.1.11
EC: 4.2.1.11
ENOLASE USP/EP/BP
ENOLASE FROM YEAST
PHOSPHOPYRUVATE HYDRATASE
enolase from bakers yeast
enolase from rabbit muscle
2-PHOSPHO-D-GLYCERATE HYDRO-LYASE
Enolase, 2-phospho-D-glycerate hydro-lyase
enolase from baker's yeast (s. cerevisiae)
Phosphopyruvate hydratase (from Yeast), 50% glycerol solution
2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase
Enolasefromrabbitmusclecrystallinesuspensionin3,2Mammoniumsulfate
Enolase from baker's yeast (S. cerevisiae),2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase
ENOLASE FROM RABBIT MUSCLE CRYSTALLINE SUSPENSION IN 3.2 M AMMONIUM SULFATE.ACTIVITY: ~30 UNITS/MG PROTEIN
[EINECS(EC#)]

232-754-3
[Molecular Formula]

NULL
[MDL Number]

MFCD00131019
Chemical PropertiesBack Directory
[Definition]

An enzyme active in glycolysis that catalyzes the conversion of 2-phosphoglyceric acid to the phosphorylated enol of pyruvic acid.
[storage temp. ]

2-8°C
[form ]

lyophilized powder
[CAS DataBase Reference]

9014-08-8
[EPA Substance Registry System]

Hydratase, phosphoenolpyruvate (9014-08-8)
Safety DataBack Directory
[Hazard Codes ]

B
[WGK Germany ]

3
Hazard InformationBack Directory
[Uses]

The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.
[General Description]

Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg2+ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes.
[Biochem/physiol Actions]

Enolase plays a key role in glycolysis and gluconeogenesis. Enolase, found in the nucleus of mammalian cells plays a key role in transcriptional modulation of genes, which participates in morphological transformation and cell proliferation.
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