| Identification | Back Directory | [Name]
EC 3.4.21.19 | [CAS]
66676-43-5 | [Synonyms]
Glu-C
PROTEASE V8
V8 PROTEASE
EC 3.4.21.19
V8 proteinase
Proteinase V8
V8 endoprotease
Glu-C, 500 u/mg
proteinase V8 VAN
Endoprotease Glu-C
PROTEASE, S AUREUS
Protease tpye ⅩⅤⅢ-B
PROTEASE TYPE XVII-B
ENDOPROTEINASE GLU-C
Protease S V8 Protease
V8protease Endoproteinase Glu-C
STAPHYLOCOCCAL SERINE PROTEINASE
EndoproteinaseGluC,Sequencinggrade
Staphylococcus aureus V8 proteinase
STAPHYLOCOCCAL SERINE PROTEASE PROTEASE V8
ENDOPROTEINASE GLU-C, STAPHYLOCOCCUS AUREUS
PROTEASE from Staphylococcus aureus V8 strain
endoproteinase glu-c from staphylococcus aureus v8
ENDOPROTEINASE GLU-C FROM*STAPHYLOCOCCUS AUREUS STR
PROTEASE TYPE XVII-B FROM*STAPHYLOCOCCUS AUREUS STR
ENDOPROTEINASE GLU-C FROM S. AUREUS STRA IN V8,~800 U/MG
Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus
Endoproteinase GluC from Staphylococcus aureus, Recombinant
Endoproteinase Glu-C from Staphylococcus aureus V8,V8 Protease
Endoproteinase Glu-C from Staphylococcus aureus (V8 proteinase), min. 2 U/mg
Endoproteinase Glu-C (V8 proteinase), MS approved froM Staphylococcus aureus lyophil.
Endoproteinase Glu-C from Staphylococcus aureus V8 Type XVII-B, lyophilized powder, 500-1,000 units/mg solid | [EINECS(EC#)]
620-751-3 | [Molecular Formula]
NULL | [MDL Number]
MFCD01324998 |
| Hazard Information | Back Directory | [Uses]
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:
- obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.
- the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
- limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
- the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.
| [Uses]
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract. | [Biochem/physiol Actions]
Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate. |
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