| Identification | Back Directory | [Name]
LUCIFERASE | [CAS]
61970-00-1 | [Synonyms]
RFLuc
Ultra-Glo
LUCIFERASE
EC 1.14.14.3
EC 1.13.12.7
Luciferase from
Einecs 263-359-4
Beetle luciferase
LUCIFERASE FIREFLY
FIREFLY LUCIFERASE
LUCIFERIN-LUCIFERASE
BACTERIAL LUCIFERASE
Ultra-Glo luciferase
Luciferase,recominant
LUCIFERASE, FROM FIREFLY
Photinus pyralis luciferase
Firefly luciferin luciferase
Luciferase, Firefly, Recomb.
LUCIFERASE, AMERICAN FIREFLY
Luciola mingrelica luciferase
LUCIFERASE (PHOTINUS PYRALIS)
Luciferase (firefly luciferin)
Luciferase (firefly recombinant)
Luciferase *Recombinant firefly*
Pyrocoelia pygidialis luciferase
Luciferase recombinant, lyophil.
luciferase from luciola mingrelica
LUCIFERASE, PHOTINUS PYRALIS, R-DNA
Luciferase Photinus pyralis (firefly)
Pyrophorus plagiophthalamus luciferase
ENLITEN(R) RLUCIFERASE/LUCIFERIN REAGENT
ALKANAL-REDUCED FMN-OXYGEN OXIDOREDUCTASE
luciferase from photinus pyralis (firefly)
PHOTINUS LUCIFERIN:OXYGEN 4-OXIDOREDUCTASE
LUCIFERASE FROM FIREFLY, WHITE POWDER, SOLUBLE*
LUCIFERASE, RECOMBINANT, FROM LUCIOLA MINGRELICA
LUCIFERASE, FROM FIREFLY WITH*TRIS-ASPAR TIC ACID
LUCIFERASE, AMERICAN FIREFLY, NATURAL, HIGH PURITY
LUCIFERASE FROM FIREFLY SOLUBLE*WITH TRI S-SUCCINIC
LUCIFERASE FROM FIREFLY, WHITE POWDER, L YOPHILIZED
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing)
LUCIFERASE, AMERICAN FIREFLY, NATURAL, STANDARD GRADE
Luciferase from Photinus pyralis (firefly), Recombinant
Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing)
Luciferase from Photinus pyralis (firefly) recombinant, expressed in E. coli, buffered aqueous solution, >=10x1010 units/mg protein | [EINECS(EC#)]
263-359-4 | [MDL Number]
MFCD00131546 |
| Hazard Information | Back Directory | [Description]
Luciferase (firefly recombinant) is a light-generating enzyme that catalyzes an oxidative reaction, using luciferin, ATP, and molecular oxygen to produce oxyluciferin, which yields visible light. The bioluminescence of this ATP-dependent luciferase is commonly used in reporter systems that are amenable to high-throughput screening. | [Uses]
Firefly luciferase is used extensively in molecular and cell biology, in particular for the efficient detection and quantitation of ATP and as a reporter for genetic function. | [Uses]
Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway. | [General Description]
Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP. | [Biochem/physiol Actions]
Firefly luciferase is a 62 kDa protein that catalyzes the production of light. The enzyme requires ATP, molecular oxygen, and the heterocyclic compound, firefly luciferin, to generate light in a two-step process. The light producing reaction is initiated by luciferin activation (adenylation of its carboxylate group) and proceeds in the presence of molecular oxygen to yield a photon of yellow-green light. |
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