| Identification | Back Directory | [Name]
CYTOCHALASIN J | [CAS]
53760-20-6 | [Synonyms]
CYTOCHALASIN J
deacetylcytochalasin H
cytochalasin J from A phomopsis species
1H-Cycloundec[d]isoindol-1-one, 2,3,3a,4,5,6,6a,9,10,11,12,15-dodecahydro-6,12,15-trihydroxy-4,10,12-trimethyl-5-methylene-3-(phenylmethyl)-, (3S,3aR,4S,6S,6aR,7E,10S,12R,13E,15R,15aR)- | [Molecular Formula]
C28H37NO4 | [MDL Number]
MFCD00077708 | [MOL File]
53760-20-6.mol | [Molecular Weight]
451.6 |
| Chemical Properties | Back Directory | [Melting point ]
138°C (dec.) | [Boiling point ]
679.5±55.0 °C(Predicted) | [density ]
1.20±0.1 g/cm3(Predicted) | [storage temp. ]
2-8°C | [solubility ]
Soluble in ethanol;Soluble in methanol;Soluble in DMSO;Soluble in dimethyl formamide | [form ]
white lyophilisate | [pka]
13.03±0.70(Predicted) | [color ]
White | [Stability:]
Light Sensitive |
| Hazard Information | Back Directory | [Uses]
Cytochalasin J is a cell-permeable fungal toxin used in actin polymerization studies and cytological research. | [Biological Activity]
cytochalasin j can alter mitotic spindle microtubule organization and kinetochore structure.the cytochalasins are cell-permeable fungal metabolites inhibiting actin polymerization, which can alter diverse processes including cell movement, growth, phagocytosis, degranulation, and secretion. | [in vitro]
cytochalasin j was identified as a deacetylated analog of cytochalasin h that weakly inhibited actin assembly but significantly altered mitotic spindle microtubule organization and kinetochore structure [1]. in a previous study, cytochalasin j treatment of prometaphase cells reduced the number of kmts and the size and organization of the kinetochore lamina. moreover, in approximately 30% of cells treated with cytochalasin j, the failure of a small number of chromosomes to attach to spindle fibers could be documented. these chromosomes showed a significant change in the organization of the kinetochore laminae. light microscopic analyses of cells treated with cytochalasin j revealed loss of chromosome congression, with chromsomes usually located at the periphery of the spindle. cells treated with 10 microg/ml cytochalasin j for 10 min and released into tissue culture medium showed a resumption of chromosome motion within a few minutes, both during congression and anaphase [2]. | [storage]
Store at -20°C | [References]
[1] walling, e. a.,krafft, g.a. and ware, b.r. actin assembly activity of cytochalasins and cytochalasin analogs assayed using fluorescence photobleaching recovery. archives of biochemistry and biophysics 264(1), 321-332 (1988).
[2] wrench, g. a. and snyder, j.a. cytochalasin j treatment significantly alters mitotic spindle microtubule organization and kinetochore structure in ptk1 cells. cell motility and the cytoskeleton 36(2), 112-124 (1997). |
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