| Identification | Back Directory | [Name]
TLCK | [CAS]
4272-74-6 | [Synonyms]
TLCK
TLCK HCL
(s)-lorid
TOS-LYS-CMK
TOSYL-LYS-CMK
TOS-LYS-CMK HCL
Tos-L-Lys-CMK·HCl
TLCK HYDROCHLORIDE
Tos-Lys-chloromethylketone
N-TOSYL-LYS-CHLOROMETHYLKETONE
TOS-LYS-CHLOROMETHYLKETONE HCL
TOSYL-L-LYSYL-CHLOROMETHANE HCL
TOSYL-L-LYSINE CHLOROMETHYLKETONE HCL
N-ALPHA-TOSYL-L-LYS CHLOROMETHYLKETONE
N-ALPHA-TOSYL-LYS-CHLOROMETHYLKETONE HCL
NALPHA-P-TOSYL-L-LYSIN CHLOROMETYL KETONE
Tosyl-L-lysyl-chloromethane hydrochloride
Nα-Tosyl-L-lysine chloroMethyl ketone HCl
1-CHLORO-3-TOSYLAMIDO-7-AMINO-L-2-HEPTANONE
N-ALPHA-P-TOSYL-L-LYSIN CHLOROMETYL KETONE HCL
L-1-CHLORO-3-TOSYLAMIDO-7-AMINO-2-HEPTANONE HCL
N-TOSYL-L-LYSINE CHLOROMETHYL KETONE HYDROCHLORIDE
N-ALPHA-TOSYL-LYS CHLOROMETHYL KETONE, HYDROCHLORIDE
1-CHLORO-3-TOSYLAMIDO-7-AMINO-2-HEPTANONE HYDROCHLORIDE
N-ALPHA-TOSYL-L-LYSINE CHLOROMETHYL KETONE HYDROCHLORIDE
1-CHLORO-3-TOSYLAMIDO-7-AMINO-L-2-HEPTANONE HYDROCHLORIDE
N-ALPHA-P-TOSYL-L-LYSINE CHLOROMETHYL KETONE HYDROCHLORIDE
Chloro-3-tosylaMido-7-aMino-2-heptanone-HCl research grade
P-TOLUENESULFONYL-L-LYSINE CHLOROMETHYL KETONE HYDROCHLORIDE
(3S)-7-AMINO-1-CHLORO-3-TOSYLAMINO-2-HEPTANONE HYDROCHLORIDE
(3S)-1-CHLORO-3-TOSYLAMIDO-7-AMINO-2-HEPTANONE HYDROCHLORIDE
L-1-CHLORO-3-(4-TOSYLAMIDO)-7-AMINO-2-HEPTANONE HYDROCHLORIDE
NALPHA-TOSYL-L-LYSINE CHLOROMETHYL KETONE HYDROCHLORIDE (TLCK)
Nα-Tosyl-L-lysine chloromethylketone hydrochloride≥ 98% (HPLC)
(3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride,98%
(3S)-1-Chloro-3-tosylaMido-7-aMino-2-heptanone hydrochloride, 98% 50MG
l-n-(5-amino-1-(chloroacetyl)pentyl)-p-toluenesulfonamidehydrochloride
n-(5-amino-1-(chloroacetyl)pentyl)-,hydrochloride,l-p-toluenesulfonamid
n-(5-amino-1-(chloroacetyl)pentyl)-4-methyl-benzenesulfonamidmonohydroch
(S)-N-[5-amino-1-(chloroacetyl)pentyl]-p-toluenesulphonamide monohydrochloride
(S)-N-(7-AMino-1-chloro-2-oxoheptan-3-yl)-4-MethylbenzenesulfonaMide hydrochloride
Benzenesulfonamide, N-(1S)-5-amino-1-(chloroacetyl)pentyl-4-methyl-, monohydrochloride
Benzenesulfonamide,N-[(1S)-5-amino-1-(2-chloroacetyl)pentyl]-4-methyl-, hydrochloride (1:1) | [EINECS(EC#)]
224-266-4 | [Molecular Formula]
C14H22Cl2N2O3S | [MDL Number]
MFCD00065395 | [MOL File]
4272-74-6.mol | [Molecular Weight]
369.31 |
| Chemical Properties | Back Directory | [Appearance]
white to light beige powder | [Melting point ]
~165 °C (dec.)
| [storage temp. ]
2-8°C
| [solubility ]
H2O: 50 mg/mL
| [form ]
powder
| [color ]
white to pink
| [optical activity]
[α]20/D 7.8±0.5°, c = 2% in H2O | [BRN ]
7106867 | [CAS DataBase Reference]
4272-74-6 |
| Hazard Information | Back Directory | [Chemical Properties]
white to light beige powder | [Uses]
Tosyllysine chloromethylketone (TLCK) is an active site-directed agent that inhibits serine proteinases with trypsin-like activity. TLCK may also act non-selectively with thiol groups and thereby inhibit cysteine proteinases and other enzymes. To prevent proteolytic degradation, TLCK may be used in protein purification protocols. TLCK selectively inactiviates clostripain obtained from C. histolyticum. | [Biological Activity]
tosyllysine chloromethyl ketone (hydrochloride) is a protease inhibitor [1][2][3].l-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-hcl (tlck) is a protease inhibitor. tlck is an active site-directed agent that inhibits serine proteinases with trypsin-like activity. tlck also interacts non-selectively with thiol groups and thereby inhibits cysteine proteinases and other enzymes. in lps-activated rat alveolar macrophages, tlck at 1-100 μm inhibited nox- accumulation and inducible inos expression in a concentration-dependent way [1]. to prevent proteolytic degradation, tlck may be used in protein purification protocols [2]. tlck significantly increased the cytotoxic activity of c. histolyticum supernatant towards human epithelial hela cells probably by hindering natural defence mechanisms of cells. 30 min incubation with bacterial supernatant increased toxicity in both concentrations (200 and 1000 μm) from 18 ± 3% to 39 ± 3% and 57 ± 8%, respectively. tlck also blocked clostripain enzymatic activity obtained from c. histolyticum. so tlck might be used to treat diseases complicated by concurrent c. histolyticum infection [3]. | [Biochem/physiol Actions]
Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli. | [storage]
Store at -20°C | [Purification Methods]
The hydrochloride slowly crystallises from a concentrated solution in absolute EtOH, thinned with EtOH/Et2O for collection and dried in vacuo. It is a suicide enzyme inhibitor of serine proteases, e.g. trypsin and clostripain. [Matsuda et al. Chem Pharm Bull Jpn 30 2512 1982, Shaw et al. Biochemistry 4 2219 1965]. | [References]
[1]. griscavage jm, wilk s, ignarro lj. serine and cysteine proteinase inhibitors prevent nitric oxide production by activated macrophages by interfering with transcription of the inducible no synthase gene. biochem biophys res commun. 1995 oct 13;215(2):721-9.
[2]. urban mk, franklin sg, zweidler a. isolation and characterization of the histone variants in chicken erythrocytes. biochemistry. 1979 sep 4;18(18):3952-60.
[3]. józwiak, j.,komar, a.,jankowska, e., et al. determination of the cytotoxic effect of clostridiumhistolyticum culture supernatant on hela cells in the presence of protease inhibitors. fems immunology & medical microbiology 45(2):137-42 (2005). |
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