HIF-1α-IN-2 is an effective HIF-1α inhibitor with anticancer potencies (IC₅₀s of 28 nM and 15 nM in MDA-MB-231 and MiaPaCa-2 cells, respectively). HIF-1α-IN-2 suppresses HIF-1α expression by blocking transcription and protein translation^[1].

HIF-1α-IN-2 (compound 7f) (5 μM; 72h) suppresses the viability of MDA-MB-231 and MiaPaCa-2 cells with IC₅₀s of 28 nM and 15 nM, respectively^[1].
HIF-1α-IN-2 (0-1 μM; 72h) suppresses the expression of HIF-1α and VEGF with dose-dependent effect^[1].
HIF-1α-IN-2 (0.25, 0.5, and 1 µM; 16-24h) can significantly inhibit the migration of MDA-MD-231 cells by 56% (0.25 µM), 83% (0.5 µM), and 85% (1 µM), respectively, and also noted in MiaPaCa-2 cells, which demonstrates the unique anti-migration effect of HIF-1α-IN-2^[1].
HIF-1α-IN-2 (0-1 μM; 72h) suppresses HIF-1α mRNA levels in MDA-MB-231 cells under hypoxia^[1].

Cell Viability Assay

Cell Viability Assay