| Identification | Back Directory | [Name]
6-CHLORO-2,3-DIOXO-1,2,3,4-TETRAHYDROQUINOXALINE, 97 | [CAS]
169-14-2 | [Molecular Formula]
C8H5ClN2O2 | [MDL Number]
MFCD00140720 |
| Safety Data | Back Directory | [Symbol(GHS) ]
GHS07 | [Hazard statements ]
H315-H319-H335 | [Precautionary statements ]
P261-P280a-P304+P340-P305+P351+P338-P405-P501a | [Safety Statements ]
26-36 | [TSCA ]
Yes | [Risk Statements ]
感受態(tài)細(xì)胞的制備是基因重組中一項(xiàng)基本技術(shù)，是獲得高質(zhì)量基因文 庫的關(guān)鍵。由于誘導(dǎo)細(xì)菌進(jìn)入感受態(tài)的機(jī)制尚不完全清楚，且感受態(tài)細(xì)胞的轉(zhuǎn) 化率受諸多因素的影響，實(shí)驗(yàn)室之間差異較大。尤其在室溫較高時(shí)，感受態(tài)制 備的效果很不穩(wěn)定，摸索高溫季節(jié)制備高效感受態(tài)細(xì)胞的方法仍十分必要。此 外，高效感受態(tài)細(xì)胞貯存不當(dāng)會(huì)很快喪失其感受性，如何較好地貯存感受態(tài)細(xì) 胞亦是一個(gè)亟待解決的問題。制備感受態(tài)細(xì)胞的方法很多，而CaCl2法是目前 實(shí)驗(yàn)室制備感受態(tài)細(xì)胞較為常用的方法之一。
圖: 感受態(tài)細(xì)胞及轉(zhuǎn)化過程圖
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