| Identification | Back Directory | [Name]
GRANZYME B | [CAS]
143180-74-9 | [Synonyms]
GZMB
GRANZYME B
EC 3.4.21.79
GRANZYME B ENZYME
GRANZYME B (HUMAN)
Granzyme B from mouse
Granzyme B from Human, Recombinant
Granzyme B from Mouse, Recombinant
Recombinant Mouse Gzmb Protein, His Tag
GRANZYME B, HUMAN, CELL CULTURE-DERIVED
SensiZyme Granzyme B Activity Assay Kit | [MDL Number]
MFCD01323415 |
| Chemical Properties | Back Directory | [storage temp. ]
-70°C | [form ]
buffered aqueous solution | [color ]
white to off-white | [biological source]
mouse | [Specific Activity]
≥40units/μg |
| Hazard Information | Back Directory | [General Description]
Granzyme B, a 247 amino acid polypeptide, contains a leader sequence, which is cleaved by a signal peptidase and a two amino acid prodomain, which is cleaved by the lysosomal cysteine protease DPPI. The recombinant granzyme B is expressed in Pichia pastoris as the mature form and appears on SDS-PAGE as a triplet (~34, 32, and 30 kDa) due to three different glycosylations. | [Biochem/physiol Actions]
Cytotoxic granules secreted by natural killer (NK) cells and cytotoxic T Lymphocytes cells are part of the mechanism used for protecting the organism from virus infected and tumor cells. Granzyme B, a serine protease, is the most prominent granzyme in a family of 11 found in the cytotoxic granules. The granzymes enter the target cell with the assistance of perforin, a critical molecule of the cytotoxic granules. In the target cell, the granzymes act on specific substrates involved with the cell death via apoptosis.Granzyme B is a neutral serine protease that cleaves aspartic acid residues, inducing cell death by various pathways. It can cleave and activate most of the caspases in vitro and in vivo resulting in a massive amplification of the caspase dependent apoptotic pathway. In addition, granzyme B cleaves directly downstream caspase substrates as PARP DNA-PK2 and DFF45/ICAD leading to cell death. This pathway bypasses inhibition of apoptosis by viral caspase inhibitors found in virus infected cells. It has been shown that granzyme B is capable of inducing cytochrome C release from the mitochondria in a caspase independent way. |
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