Hazard Information | Back Directory | [Description]
2-Aminopurine is a fluorescent analog of guanosine and adenosine that is used as a site-specific probe of nucleic acid structure and dynamics.1 It base pairs with cytosine in a wobble configuration or with thymine in a Watson–Crick geometry.1 In addition to its usefulness in studies of base stacking interactions, 2-aminopurine has been used as a probe for DNA base flipping by methyl transferases.2 2-Aminopurine exhibits an excitation/emission of 320/381 nm, respectively, when adjusted to reduce interference with DNA base absorption as well as tryptophan fluorescence.2 2-Aminopurine inhibits the double-stranded RNA-dependent protein kinase, protein kinase R, whose activity mediates antiviral defense and participates in toll-like receptor signaling.3 | [Uses]
2-Aminopurine Dihydrochloride is a probe of structural dynamics and charge transfer in DNA. | [storage]
Store at -20°C | [References]
1. Jean, J.M., and Hall, K.B. 2-Aminopurine fluorescence quenching and lifetimes: Role of base stacking Proc. Natl. Acad. Sci. USA 98(1),37-41(2001). 2. Holz, B., Klimasauskas, S., Serva, S., et al. 2-Aminopurine as a fluorescent probe for DNA base flipping by methyltransferases Nucleic Acids Res. 26(4),1076-1083(1998). 3. Endoh, Y., Chung, Y.M., Clark, I.A., et al. IL-10-dependent S100A8 gene induction in monocytes/macrophages by double-stranded RNA J. Immunol. 182(4),2258-2268(2009). |
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